FastQCFastQC Report
Tue 31 May 2016
SRR212490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99791
Sequences flagged as poor quality0
Sequence length58
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT36313.638604683789119No Hit
ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG13601.362848353057891No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC11291.1313645519134992No Hit
ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC10411.0431802467156357No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.5772063612951067No Hit
ATTCCAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5600.5611728512591315No Hit
ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA5550.5561623793728894No Hit
ATTCCAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5140.5150765099057029No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATTCCAGGGAAGCAGTGG5060.5070597548877154No Hit
ATTCCAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4800.4810053010792557No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC3410.3417141826417212No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.3417141826417212No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.32868695573749135No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.3096471625697708No Hit
ATTCCAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2780.2785822368750689No Hit
ATTCCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1760.17636861039572707No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1580.158330911605255Illumina Single End Adapter 2 (96% over 25bp)
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1530.15332043971901274No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC1430.14329949594652824No Hit
ATTCCAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.12125341964706235No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG1150.11524085338357166No Hit
ATTCCAGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT1120.11223457025182633No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.10722409836558407No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT1060.10622200398833562No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT1060.10622200398833562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCA150.007010678852.3529139
GGATGGA203.319531E-452.3529138
GGGTTAC353.635978E-852.3529137
GACAGTA307.5591925E-752.3529139
GATGTAC150.007010678852.3529139
AGGGCAA251.5795968E-552.3529136
GGACATA150.007010678852.3529138
GAGCTGT251.5795968E-552.3529139
TAGTCGA150.007010678852.35291310
GAATGGT401.7571438E-952.3529139
GGGAGTA307.5591925E-752.3529137
ATGTGGC150.007010678852.35291310
GCAGCGA150.007010678852.3529139
GGTAGTC203.319531E-452.3529138
GACTGTA307.5591925E-752.3529139
GAATATC150.007010678852.3529139
AATATCA150.007010678852.35291310
GGGCGCA307.5591925E-752.3529137
GGTACCT150.007010678852.3529138
GGCAGCG150.007010678852.3529138
Produced by FastQC (version 0.11.5)