FastQCFastQC Report
Tue 31 May 2016
SRR212489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97035
Sequences flagged as poor quality0
Sequence length56
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT43394.471582418714896No Hit
ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA14171.4602978306796517No Hit
ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA9260.9542948420672954No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.7100530736332251No Hit
ATTCCAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT5970.6152419230174679No Hit
ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG4710.4853918689132787No Hit
ATTCCAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA4600.474055753078786No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATTCCAGGGAAGCAGT4540.46787241716906275No Hit
ATTCCAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4450.4585974133044778No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC3980.4101612820116453No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.3874890503426599No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.3843973823877982No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.34729736692945845No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC2460.25351677229865516No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT2250.2318750966146236No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2010.2071417529757304No Hit
ATTCCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1910.19683619312619158No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1410.14530839387849745Illumina Single End Adapter 2 (95% over 23bp)
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1360.14015561395372803No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.14015561395372803No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA1250.12881949811923535No Hit
ATTCCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT1150.1185139382696965No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.11439171432988096No Hit
ATTCCAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.11439171432988096No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA1100.11336115834492709No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT1050.10820837842015767No Hit
ATTCCAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.10614726645024992No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT1000.10305559849538826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCC251.9892774E-550.35000250
AAGGAGT150.00817899650.35000250
GGTATGG150.00817899650.35000250
GAAGGAG150.00817899650.35000249
TACAGCC204.0237172E-450.3550
AGGCCGC1750.050.3549
CGTACAG204.0237172E-450.3548
GGTAGGC204.0237172E-450.3548
CAGGCCG1750.050.3548
GGGTAGG204.0652903E-450.24532347
GCAGGCC1950.050.24532347
CGCAGGC1900.050.24532346
TCGTACA252.0139494E-550.24532347
CTCGTAC252.0139494E-550.24532346
TAGCGCA1350.050.2192243
AGGATTT150.0082635750.2192244
CAACCTC252.0201569E-550.2192242
GCGGCAT252.0201569E-550.2192242
TGCGAAA204.0757368E-450.2192244
AACCTCG252.0201569E-550.2192243
Produced by FastQC (version 0.11.5)