FastQCFastQC Report
Tue 31 May 2016
SRR212488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87897
Sequences flagged as poor quality0
Sequence length56
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT37214.233364051105271No Hit
ATTCCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA14071.6007372265265027No Hit
ATTCCAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA9561.087636665642741No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.6678271158287541No Hit
ATTCCAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT5590.6359716486341969No Hit
ATTCCAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG5000.5688476284742369No Hit
ATTCCAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA4520.5142382561407102No Hit
ATTCCAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4150.4721435316336166No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.456215798036338No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC3860.43915036918211087No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.38567869210553263No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.34358396759843907No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATTCCAGGGAAGCAGT2890.3287939292581089No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC2460.27987303320932455No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT2290.2605322138412005No Hit
ATTCCAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1600.1820312411117558No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1590.18089354585480732No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1310.14903807866025007No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.14790038340330158No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1250.14221190711855922Illumina Single End Adapter 2 (95% over 23bp)
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA1160.13197264980602297No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT1090.12400878300738365No Hit
ATTCCAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.12173339249348669No Hit
ATTCCAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.12173339249348669No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.11945800197958975No Hit
ATTCCAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC1050.11945800197958975No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA1040.11832030672264127No Hit
ATTCCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT1030.1171826114656928No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC960.10921874466705349No Hit
ATTCCAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.10694335415315653No Hit
ATTCCAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCCGGCCGCC930.10580565889620806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCCT150.00823545950.25847647
TCCTTGG150.00823545950.25847650
TGTCCTT150.00823545950.25847648
GGTCTTC150.00823545950.25847648
GGCCGCT700.050.25847650
GGCTGCT150.00823545950.25847648
GTCCTTG150.00823545950.25847649
GCACGGT150.00823545950.25847647
ATTCGTA301.0004387E-650.25847650
CGGGAAG150.00823545950.25847646
TTGATCC150.00823545950.25847650
GGTAGGC150.00823545950.25847648
AGCACGG150.00823545950.25847646
CTTGTCC150.00823545950.25847646
GGTATGG150.00823545950.25847650
CGCCGCC150.00823545950.25847646
GGGCTGC204.057896E-450.25847247
CCGTGCG204.080974E-450.20080641
TTTCTGG150.00827294750.20080642
ATGGCCT150.00827294750.20080641
Produced by FastQC (version 0.11.5)