FastQCFastQC Report
Tue 31 May 2016
SRR212487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33715
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT11473.4020465668100255No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7612.2571555687379505No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6321.8745365564288894No Hit
TTTAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5831.7292006525285482No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5281.5660685154975529No Hit
TTTAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3791.1241287260863118No Hit
TTTAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3571.0588758712739137No Hit
TTTAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3381.0025211330268426No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2670.7919323743141036No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2370.7029512086608335No Hit
TTTAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1590.4716001779623313No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1480.4389737505561323No Hit
TTTAGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1390.41227940086015125No Hit
TTTAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1350.40041524543971524No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1350.40041524543971524No Hit
TTTAGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1290.38261901230906126No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1120.3321963517722082No Hit
TTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1020.3025359632211182No Hit
TTTAGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1000.2966038855109002No Hit
TTTAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA990.2936378466557912No Hit
TTTAGGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC970.2877057689455732No Hit
TTTAGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG880.26101141924959215No Hit
TTTAGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG860.25507934153937417No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC850.2521133026842652No Hit
TTTAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT800.23728310840872013No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC800.23728310840872013No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC790.23431706955361117No Hit
TTTAGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG770.22838499184339314No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT770.22838499184339314No Hit
TTTAGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA770.22838499184339314No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.22245291413317514No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA750.22245291413317514No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC740.21948687527806615No Hit
TTTAGGGGGGCTTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG700.20762271985763012No Hit
TTTAGGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC700.20762271985763012No Hit
TTTAGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT680.20169064214741214No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA680.20169064214741214No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.19872460329230313No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC660.1957585644371941No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA660.1957585644371941No Hit
TTTAGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC650.19279252558208512No Hit
TTTAGGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT640.1898264867269761No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA620.18389440901675813No Hit
TTTAGGGGGTGATCAGAAGTTCACCAGCATCCTTAGCTGCCGAGCAAGGATGAGGCCA620.18389440901675813No Hit
TTTAGGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT620.18389440901675813No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA600.17796233130654013No Hit
TTTAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG600.17796233130654013No Hit
TTTAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG600.17796233130654013No Hit
TTTAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA590.1749962924514311No Hit
TTTAGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT580.1720302535963221No Hit
TTTAGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG560.1660981758861041No Hit
TTTAGGGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT560.1660981758861041No Hit
TTTAGGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA540.1601660981758861No Hit
TTTAGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA520.15423402046566811No Hit
TTTAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC510.1512679816105591No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC500.1483019427554501No Hit
TTTAGGGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA490.1453359039003411No Hit
TTTAGGGGGAGTTAAAAAAATCCATGAGAACGTTTTCTAGGATTTCCATTTTCATTGC490.1453359039003411No Hit
TTTAGGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA480.14236986504523208No Hit
TTTAGGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC470.1394038261901231No Hit
TTTAGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC470.1394038261901231No Hit
TTTAGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAG460.13643778733501408No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG460.13643778733501408No Hit
TTTAGGGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC460.13643778733501408No Hit
TTTAGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT450.1334717484799051No Hit
TTTAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA450.1334717484799051No Hit
TTTAGGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG450.1334717484799051No Hit
TTTAGGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA440.13050570962479607No Hit
TTTAGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC430.12753967076968709No Hit
TTTAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT430.12753967076968709No Hit
TTTAGGGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGACA420.12457363191457808No Hit
TTTAGGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA420.12457363191457808No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA420.12457363191457808No Hit
TTTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT420.12457363191457808No Hit
TTTAGGGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA400.11864155420436007No Hit
TTTAGGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC400.11864155420436007No Hit
TTTAGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACTT390.11567551534925108No Hit
TTTAGGGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC390.11567551534925108No Hit
TTTAGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG390.11567551534925108No Hit
TTTAGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAAG390.11567551534925108No Hit
TTTAGGGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGCCATGTC390.11567551534925108No Hit
TTTAGGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA380.11270947649414206No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA380.11270947649414206No Hit
TTTAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCAC380.11270947649414206No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA370.10974343763903308No Hit
TTTAGGGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCACGC360.10677739878392407No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCAGGGTAGAGAGGAGCGTGGCCTTCTCCT360.10677739878392407No Hit
TTTAGGGGGGAGAGGATCAAGCTGCTTTTGCCTCCCAAGCGCTGGGATTTAAGGAGAC350.10381135992881506No Hit
TTTAGGGGGATTCTTACATCTTTTCCTTGCCCTCTGTGCTTTGAAAGCACTGAATAAA340.10084532107370607No Hit
TTTAGGGGGGGCTTGCTAGATATCAGTTGCCTTCTTTTGTTCAGCTAAGGAACTTGAA340.10084532107370607No Hit
TTTAGGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC340.10084532107370607No Hit
TTTAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG340.10084532107370607No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA340.10084532107370607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGAGA700.052.00000418
GATGGGT150.00715979452.041
ATCCTGC251.6208727E-552.020
CAGCTAA150.00715979452.042
ATCCCCA150.00715979452.024
GTTGATC251.6208727E-552.016
TAGCGCT150.00715979452.026
AACGTGC150.00715979452.048
TTGTTCA150.00715979452.037
GAGAAAG650.052.021
CTGAGTT150.00715979452.024
CATTGGC150.00715979452.049
CAGCATT251.6208727E-552.043
CAGTTGC150.00715979452.024
GTCACCA150.00715979452.046
TTCGTGG650.052.015
TTCGTAG150.00715979452.029
GATATGG251.6208727E-552.051
GCTAGCG150.00715979452.024
GCTAGAT150.00715979452.015
Produced by FastQC (version 0.11.5)