FastQCFastQC Report
Tue 31 May 2016
SRR212486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35502
Sequences flagged as poor quality0
Sequence length58
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT10823.0477156216551182No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7792.194242577882936No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6571.8505999661990873No Hit
TTTAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6551.844966480761647No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5831.6421610050138022No Hit
TTTAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3621.0196608641766662No Hit
TTTAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3320.935158582615064No Hit
TTTAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3120.8788237282406625No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2720.7661540194918597No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2310.6506675680243367No Hit
TTTAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1740.4901132330572926No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1670.4703960340262521No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1410.3971607233395302No Hit
TTTAGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1370.38589375246464985No Hit
TTTAGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1370.38589375246464985No Hit
TTTAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1340.37744352430848965No Hit
TTTAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1140.3211086699340882No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1000.2816742718720072No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA980.2760407864345671No Hit
TTTAGGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC980.2760407864345671No Hit
TTTAGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC980.2760407864345671No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC970.273224043715847No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC970.273224043715847No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC960.2704073009971269No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA940.26477381555968676No Hit
TTTAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT900.2535068446848065No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.25069010196608643No Hit
TTTAGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA880.24787335924736634No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA820.2309729029350459No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC810.22815616021632587No Hit
TTTAGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG810.22815616021632587No Hit
TTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT800.22533941749760578No Hit
TTTAGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT780.21970593206016562No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC750.2112557039040054No Hit
TTTAGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG740.20843896118528535No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.20843896118528535No Hit
TTTAGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG730.20562221846656525No Hit
TTTAGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC730.20562221846656525No Hit
TTTAGGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA720.2028054757478452No Hit
TTTAGGGGGTGATCAGAAGTTCACCAGCATCCTTAGCTGCCGAGCAAGGATGAGGCCA720.2028054757478452No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC700.19717199031040505No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA690.194355247591685No Hit
TTTAGGGGGGCTTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG680.19153850487296492No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA670.18872176215424483No Hit
TTTAGGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC650.18308827671680467No Hit
TTTAGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT640.1802715339980846No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC630.17745479127936453No Hit
TTTAGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG630.17745479127936453No Hit
TTTAGGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA620.17463804856064447No Hit
TTTAGGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA590.16618782040448427No Hit
TTTAGGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT590.16618782040448427No Hit
TTTAGGGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA580.16337107768576417No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG570.1605543349670441No Hit
TTTAGGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT570.1605543349670441No Hit
TTTAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG570.1605543349670441No Hit
TTTAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG560.15773759224832404No Hit
TTTAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA550.15492084952960397No Hit
TTTAGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC550.15492084952960397No Hit
TTTAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC550.15492084952960397No Hit
TTTAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA540.1521041068108839No Hit
TTTAGGGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT520.14647062137344374No Hit
TTTAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCAC510.14365387865472368No Hit
TTTAGGGGGATGCCTGCTGGGGAAATGCAAAGCAGAGGTAGAAGTGTAGCAGCCACCA510.14365387865472368No Hit
TTTAGGGGGAGTTAAAAAAATCCATGAGAACGTTTTCTAGGATTTCCATTTTCATTGC500.1408371359360036No Hit
TTTAGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAG480.13520365049856345No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCAGGGTAGAGAGGAGCGTGGCCTTCTCCT480.13520365049856345No Hit
TTTAGGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC470.13238690777984338No Hit
TTTAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG470.13238690777984338No Hit
TTTAGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGG460.12957016506112332No Hit
TTTAGGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA460.12957016506112332No Hit
TTTAGGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC450.12675342234240325No Hit
TTTAGGGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC440.12393667962368317No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA430.1211199369049631No Hit
TTTAGGGGGGCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTAC430.1211199369049631No Hit
TTTAGGGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC430.1211199369049631No Hit
TTTAGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC420.11830319418624302No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA420.11830319418624302No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA420.11830319418624302No Hit
TTTAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT410.11548645146752295No Hit
TTTAGGGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG410.11548645146752295No Hit
TTTAGGGGGGAGAGGATCAAGCTGCTTTTGCCTCCCAAGCGCTGGGATTTAAGGAGAC400.11266970874880289No Hit
TTTAGGGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA390.10985296603008281No Hit
TTTAGGGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGACA390.10985296603008281No Hit
TTTAGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA390.10985296603008281No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA380.10703622331136274No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA380.10703622331136274No Hit
TTTAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACGG370.10421948059264267No Hit
TTTAGGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAAA370.10421948059264267No Hit
TTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG370.10421948059264267No Hit
TTTAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCGT370.10421948059264267No Hit
TTTAGGGGGGGAGGCAAAGGGGCCCTGCTACCGAGTGCTGCATGATGGCGGATGCTCG370.10421948059264267No Hit
TTTAGGGGGGCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAG360.1014027378739226No Hit
TTTAGGGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGCCATGTC360.1014027378739226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCA150.00713744252.04660824
AGCCCAT150.00713744252.04660851
TGCATGT203.3871562E-452.04660824
TCCTTAG150.00713744252.04660830
TGGCAGT150.00713744252.04660842
GCAAGGA150.00713744252.04660844
GATATGG251.613978E-552.04660851
CCAGCAT150.00713744252.04660824
CGCCCAG203.3871562E-452.04660852
AGTAGCA150.00713744252.04660828
TATGCTT150.00713744252.04660835
GTTCCTG150.00713744252.04660827
TGCCGAG150.00713744252.04660838
GCAGGTG150.00713744252.04660840
CATCCTT150.00713744252.04660828
GAAGTGA150.00713744252.04660818
AGAGGCG150.00713744252.04660850
TGATCCT150.00713744252.04660818
TCGAACT203.3871562E-452.04660852
GCGAATG150.00713744252.04660846
Produced by FastQC (version 0.11.5)