FastQCFastQC Report
Tue 31 May 2016
SRR212485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36353
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT10492.885594036255605No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7382.030093802437213No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6951.9118092041922263No Hit
TTTAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6141.6889940307539955No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5101.4029103512777488No Hit
TTTAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3761.034302533491046No Hit
TTTAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3390.9325227629081506No Hit
TTTAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2940.8087365554424668No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2870.7894809231700273No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2080.5721673589524936No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1450.3988666685005364No Hit
TTTAGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1430.39336505927983934No Hit
TTTAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1400.38511264544879376No Hit
TTTAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1250.34385057629356586No Hit
TTTAGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1180.32459494402112615No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1150.31634253019008063No Hit
TTTAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1030.2833328748658983No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA980.26957885181415564No Hit
TTTAGGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC980.26957885181415564No Hit
TTTAGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC950.26132643798311006No Hit
TTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT940.2585756333727615No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC920.2530740241520645No Hit
TTTAGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA920.2530740241520645No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC870.23932000110032187No Hit
TTTAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT860.23656919648997332No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA830.22831678265892774No Hit
TTTAGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG820.2255659780485792No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC810.2228151734382307No Hit
TTTAGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT740.20355954116579097No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA730.20080873655544249No Hit
TTTAGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG730.20080873655544249No Hit
TTTAGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG730.20080873655544249No Hit
TTTAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG730.20080873655544249No Hit
TTTAGGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT720.19805793194509394No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT720.19805793194509394No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA720.19805793194509394No Hit
TTTAGGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA710.1953071273347454No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC700.19255632272439688No Hit
TTTAGGGGGTGATCAGAAGTTCACCAGCATCCTTAGCTGCCGAGCAAGGATGAGGCCA700.19255632272439688No Hit
TTTAGGGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA690.18980551811404836No Hit
TTTAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA680.18705471350369982No Hit
TTTAGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC680.18705471350369982No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.1843039088933513No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG650.17880229967265424No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC630.1733006904519572No Hit
TTTAGGGGGGCTTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG600.16504827662091162No Hit
TTTAGGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC600.16504827662091162No Hit
TTTAGGGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT600.16504827662091162No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC580.15954666740021456No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC580.15954666740021456No Hit
TTTAGGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA560.1540450581795175No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.15129425356916898No Hit
TTTAGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC540.14854344895882046No Hit
TTTAGGGGGAGTTAAAAAAATCCATGAGAACGTTTTCTAGGATTTCCATTTTCATTGC540.14854344895882046No Hit
TTTAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC540.14854344895882046No Hit
TTTAGGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC530.14579264434847192No Hit
TTTAGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC530.14579264434847192No Hit
TTTAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT530.14579264434847192No Hit
TTTAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA520.1430418397381234No Hit
TTTAGGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA520.1430418397381234No Hit
TTTAGGGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA510.14029103512777488No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA510.14029103512777488No Hit
TTTAGGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT510.14029103512777488No Hit
TTTAGGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT510.14029103512777488No Hit
TTTAGGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA500.13754023051742634No Hit
TTTAGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA500.13754023051742634No Hit
TTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG490.13478942590707782No Hit
TTTAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG480.13203862129672927No Hit
TTTAGGGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGACA470.12928781668638076No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA460.12653701207603224No Hit
TTTAGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG460.12653701207603224No Hit
TTTAGGGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGCCATGTC460.12653701207603224No Hit
TTTAGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAG450.12378620746568371No Hit
TTTAGGGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC450.12378620746568371No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA450.12378620746568371No Hit
TTTAGGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG440.12103540285533518No Hit
TTTAGGGGGGCTTGGCGTGATTCCGTCCTGCGCGTCTGTTCTGTGGAACAGGAGGCAG430.11828459824498666No Hit
TTTAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG430.11828459824498666No Hit
TTTAGGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA420.11553379363463813No Hit
TTTAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCAC420.11553379363463813No Hit
TTTAGGGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGA410.1127829890242896No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCAGGGTAGAGAGGAGCGTGGCCTTCTCCT410.1127829890242896No Hit
TTTAGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT390.10728137980359255No Hit
TTTAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTGA390.10728137980359255No Hit
TTTAGGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC390.10728137980359255No Hit
TTTAGGGGGGAGAGGATCAAGCTGCTTTTGCCTCCCAAGCGCTGGGATTTAAGGAGAC390.10728137980359255No Hit
TTTAGGGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTCTGGTATT380.10453057519324402No Hit
TTTAGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG370.10177977058289549No Hit
TTTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT370.10177977058289549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACAG203.368168E-452.1091238
ACTCCTG203.368168E-452.1091240
GCAGCAT251.6032827E-552.1091242
AGCATTT251.6032827E-552.1091244
AGACTCC203.368168E-452.1091238
ACAGGCG203.368168E-452.1091241
TCCCCAA251.6032827E-552.1091225
CACATTT150.007104941652.10911626
CAGCATT307.6686047E-752.10911643
TGCGCCT150.007104941652.10911642
CGCCTGT150.007104941652.10911628
GTCGCGT150.007104941652.10911644
CACAATT150.007104941652.10911642
GCGGCGA150.007104941652.10911629
TTATCAC150.007104941652.10911638
CAATTAT150.007104941652.10911644
CACGAGT150.007104941652.10911639
CCCACAT150.007104941652.10911624
ACATTTT150.007104941652.10911627
CATCAAC150.007104941652.10911626
Produced by FastQC (version 0.11.5)