SRR212484_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212484_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49802
Sequences flagged as poor quality	0
Sequence length	56
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	3233	6.4917071603550065	No Hit
AAAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1127	2.262961326854343	No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	920	1.8473153688606883	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	914	1.835267659933336	No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	464	0.9316894903819123	No Hit
AAAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	457	0.917633829966668	No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	298	0.5983695433918317	No Hit
AAAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	260	0.5220673868519337	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGT	185	0.3714710252600297	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	181	0.3634392193084615	No Hit
AAAACGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	180	0.36143126782056945	No Hit
AAAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152	0.305208626159592	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	146	0.2931609172322397	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	134	0.26906549937753504	Illumina Single End Adapter 2 (95% over 23bp)
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC	127	0.2550098389622907	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	122	0.2449700815228304	Illumina Single End Adapter 2 (96% over 30bp)
AAAACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117	0.23493032408337017	No Hit
AAAACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	102	0.20481105176498934	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC	101	0.2028031002770973	No Hit
AAAACGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	100	0.20079514878920526	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGG	94	0.18874743986185294	No Hit
AAAACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.1867394883739609	No Hit
AAAACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	93	0.1867394883739609	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT	89	0.17870768242239268	No Hit
AAAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	88	0.17669973093450062	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT	82	0.1646520220071483	No Hit
AAAACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.15862816754347214	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC	78	0.1566202160555801	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	72	0.14457250712822778	No Hit
AAAACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	69	0.13854865266455163	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	69	0.13854865266455163	Illumina Single End Adapter 2 (100% over 29bp)
AAAACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67	0.13453274968876752	No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCG	55	0.11043733183406289	No Hit
AAAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54	0.10842938034617085	No Hit
AAAACGGGGTTCTCTCTTCAATTACTGGTGAATTGTTAACAGGTAGATTTGTTTTT	53	0.10642142885827878	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC	51	0.10240552588249467	Illumina Single End Adapter 2 (96% over 28bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCAAT	25	1.9639268E-5	50.397762	49
TTGTATG	15	0.008124884	50.397762	50
TGGTATG	20	3.9857064E-4	50.397762	50
GCCGCTC	20	3.9857064E-4	50.397762	50
TGGCGCT	15	0.008124884	50.397762	47
TTTGTAT	15	0.008124884	50.397762	49
GTTGCAA	25	1.9639268E-5	50.397762	48
CATCGAA	50	5.456968E-12	50.397762	50
TCGTTGC	25	1.9758048E-5	50.346546	46
GATTTGT	15	0.008157653	50.346546	46
GCATTTT	25	1.9877421E-5	50.295433	45
AGATTTG	15	0.008190519	50.295433	45
TCTCGTT	25	1.9997393E-5	50.244423	44
GTCTCGT	25	1.9997393E-5	50.244423	43
AGCCGTC	25	1.9997393E-5	50.244423	39
CGTCTCG	25	1.9997393E-5	50.244423	42
GTAGATT	15	0.008223484	50.24442	43
ACGTAGA	15	0.008223484	50.24442	41
CGTAGAG	15	0.008223484	50.24442	42
GAGCATT	15	0.008223484	50.24442	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content