##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212484_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 49802 Sequences flagged as poor quality 0 Sequence length 56 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.01269025340348 39.0 38.0 40.0 35.0 40.0 2 37.96134693385808 39.0 38.0 40.0 35.0 40.0 3 37.951949720894746 39.0 38.0 40.0 35.0 40.0 4 37.98839404039998 39.0 38.0 40.0 35.0 40.0 5 37.61328862294687 39.0 38.0 40.0 33.0 40.0 6 37.59999598409703 39.0 38.0 40.0 33.0 40.0 7 37.91632866149954 39.0 38.0 40.0 35.0 40.0 8 37.8143448054295 39.0 38.0 40.0 35.0 40.0 9 37.66111802738846 39.0 38.0 40.0 35.0 40.0 10 36.619171920806394 39.0 35.0 40.0 31.0 40.0 11 36.42373800248986 38.0 35.0 40.0 31.0 40.0 12 36.394582546885665 38.0 35.0 40.0 31.0 40.0 13 36.28820127705715 38.0 35.0 40.0 30.0 40.0 14 36.29360266655958 38.0 35.0 40.0 30.0 40.0 15 36.349985944339586 38.0 36.0 40.0 31.0 40.0 16 36.0014256455564 38.0 36.0 40.0 30.0 40.0 17 36.23195855588129 38.0 36.0 40.0 31.0 40.0 18 36.02582225613429 38.0 36.0 40.0 30.0 40.0 19 35.32860126099354 38.0 35.0 40.0 29.0 40.0 20 35.79613268543432 38.0 35.0 40.0 29.0 40.0 21 35.5919240191157 38.0 35.0 40.0 29.0 40.0 22 35.33837998473957 38.0 35.0 40.0 29.0 40.0 23 34.945042367776395 38.0 35.0 39.0 27.0 40.0 24 34.92919963053693 38.0 35.0 39.0 27.0 40.0 25 34.97789245411831 38.0 35.0 40.0 27.0 40.0 26 34.4557045901771 38.0 35.0 39.0 26.0 40.0 27 34.465061644110676 38.0 35.0 39.0 26.0 40.0 28 33.46369623709891 38.0 33.0 39.0 22.0 40.0 29 33.76450744950002 38.0 34.0 39.0 23.0 40.0 30 32.98755070077507 38.0 33.0 39.0 17.0 40.0 31 31.851773021163808 38.0 32.0 39.0 2.0 40.0 32 30.798481988675153 37.0 29.0 39.0 2.0 40.0 33 31.495241154973694 38.0 31.0 39.0 2.0 40.0 34 31.40536524637565 38.0 31.0 39.0 2.0 40.0 35 30.24663668125778 37.0 29.0 39.0 2.0 40.0 36 29.282679410465445 37.0 26.0 39.0 2.0 40.0 37 28.58851050158628 36.0 22.0 39.0 2.0 40.0 38 28.545881691498334 36.0 21.0 39.0 2.0 40.0 39 28.428998835388136 36.0 19.0 39.0 2.0 40.0 40 28.138669129753826 36.0 18.0 39.0 2.0 40.0 41 27.916690092767357 36.0 12.0 39.0 2.0 40.0 42 27.845327496887677 36.0 9.0 39.0 2.0 40.0 43 27.65493353680575 35.0 6.0 39.0 2.0 40.0 44 27.254146419822497 35.0 2.0 39.0 2.0 40.0 45 27.212742460142163 35.0 2.0 39.0 2.0 40.0 46 26.82123207903297 35.0 2.0 39.0 2.0 40.0 47 26.698767117786435 35.0 2.0 39.0 2.0 40.0 48 26.554736757559937 35.0 2.0 39.0 2.0 40.0 49 26.33500662623991 35.0 2.0 39.0 2.0 40.0 50 26.0569655837115 35.0 2.0 39.0 2.0 40.0 51 25.56732661338902 35.0 2.0 39.0 2.0 40.0 52 25.294024336372033 35.0 2.0 39.0 2.0 40.0 53 24.8530179510863 35.0 2.0 39.0 2.0 40.0 54 24.584695393759286 35.0 2.0 39.0 2.0 40.0 55 24.053471748122565 34.0 2.0 39.0 2.0 40.0 56 13.607023814304647 17.0 2.0 22.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.2755653598140029 1 2 -0.4984689698433087 1 3 -0.18352893342557053 1 4 -0.08269707719208697 1 5 -0.5375317597280826 1 6 -0.6309784328240369 1 7 -0.6479825973737405 1 8 -0.999088831197362 1 9 -0.976359843529174 1 10 -0.08083430145516957 1 11 -0.43445511712189244 1 12 -0.561381490818043 1 13 -0.3069806219795268 1 14 -1.242210729933646 1 15 -1.1297669784677922 1 16 -1.0727433646583648 1 17 -0.4880546744399865 1 18 -0.9966151123437115 1 19 -1.7008818783935382 1 20 -0.48650132430228865 1 21 -1.3098814832587138 1 22 -1.0355522401730255 1 23 -1.8431770359642528 1 24 -1.2510269185587717 1 25 -1.1314719363815655 1 26 -0.7439298761991466 1 27 0.047124216092562676 1 28 -0.633939494334939 1 29 -1.2110477400691337 1 30 -1.1810690651167697 1 31 -2.340746262180147 1 32 -2.754195559744254 1 33 -3.6682174453987777 1 34 -3.461492237114758 1 35 -4.733014874105258 1 36 -6.175636429857626 1 37 -4.964896912340063 1 38 -6.308630404932284 1 39 -6.1621150853268745 1 40 -6.044871383517606 1 41 -5.721560289560024 1 42 -5.898882974457621 1 43 -5.180833004514838 1 44 -6.170844962425875 1 45 -6.194626673834438 1 46 -6.19430656055275 1 47 -6.869220348804568 1 48 -5.939781997444861 1 49 -5.590132321366628 1 50 -4.883912061674835 1 51 -4.09449137635082 1 52 -4.355112512304974 1 53 -4.041231280301304 1 54 -3.926370418806197 1 55 -3.0465029749794432 1 56 -0.9290617892756359 2 1 -0.6513100406650665 2 2 -1.221447693247569 2 3 -0.34310340151067464 2 4 -0.9912077154899634 2 5 -0.8937019724940427 2 6 -1.0620422626112713 2 7 -1.058195363331187 2 8 -1.3199398950271473 2 9 -1.1346577158696007 2 10 -1.7101960035828299 2 11 -3.8944551171218933 2 12 -3.986062341881869 2 13 -4.0605976432561235 2 14 -3.6396575384442826 2 15 -3.8174265529358777 2 16 -3.554871024232831 2 17 -4.172309993588925 2 18 -2.7174661761734953 2 19 -2.9774776230743925 2 20 -2.4090545157916523 2 21 -1.5298814832587126 2 22 -0.8355522401730227 2 23 -0.9929642700068086 2 24 -1.2003886206864323 2 25 -1.320833638509228 2 26 -1.7205256208800037 2 27 -1.1703225924180742 2 28 -1.2943650262498352 2 29 -1.792324335813813 2 30 -0.9802180012869819 2 31 -0.8832994536695082 2 32 0.8300597594046835 2 33 -0.052472764547715656 2 34 -0.6508539392424169 2 35 -0.11939785282866211 2 36 0.35968271907854543 2 37 -0.3789394655315519 2 38 -0.6358644474854742 2 39 -0.29700870234815113 2 40 -0.6895522345814342 2 41 -0.4326241193472562 2 42 0.1202659617125903 2 43 -0.2923223662169683 2 44 -2.0942492177450234 2 45 -2.0580309291535883 2 46 -0.5470725179995597 2 47 -1.0334756679535069 2 48 -0.8938245506363494 2 49 -0.9918344490262001 2 50 -2.808592912738664 2 51 -4.997044567840181 2 52 -4.558091235709231 2 53 -4.9803802164715165 2 54 -4.521689567742367 2 55 -3.4984178685964658 2 56 -3.057146895658615 3 1 -0.37131004066506534 3 2 -0.38144769324756567 3 3 -0.2778860102063234 3 4 -0.391207715489962 3 5 -0.7997889290157829 3 6 -0.7646509582634451 3 7 -1.0355866676790129 3 8 -0.6364616341575839 3 9 -1.136396846304379 3 10 -1.548456873148048 3 11 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2.99863573751308 6 46 3.863760815333773 6 47 3.6015243320464947 6 48 3.9928421160303174 6 49 3.673165550973799 6 50 3.8714070872613355 6 51 2.6337887654931507 6 52 2.7827420976241015 6 53 3.1187864501951523 6 54 3.1724770989243005 6 55 4.025748798070204 6 56 2.977853104341385 7 1 0.47651604629145794 7 2 0.031595785013301736 7 3 0.3742879028371533 7 4 0.39140098016221003 7 5 0.6132545492450916 7 6 0.2570881721713363 7 7 -0.2747171024616222 7 8 -0.027765981983669974 7 9 0.1244727189130117 7 10 -0.6788916557567433 7 11 -0.5431507692958064 7 12 -0.17562755927317397 7 13 0.10375018283082937 7 14 -0.26487492974863613 7 15 -1.1052526398923987 7 16 0.31208549750630254 7 17 -0.15317955880631473 7 18 0.2138381716525899 7 19 0.4790441160560448 7 20 -0.22470668970469632 7 21 1.124901125436935 7 22 1.051404281566107 7 23 1.631383556080145 7 24 1.063959205400522 7 25 -0.03387711677009264 7 26 0.5012135095547805 7 27 0.28532958149497034 7 28 1.243026278097993 7 29 0.48506696853401365 7 30 1.4345646074086673 7 31 1.5949614158957104 7 32 3.4109293246220744 7 33 1.9492663658870661 7 34 2.853493886844536 7 35 4.095384755866988 7 36 4.905769675600286 7 37 3.388017056207577 7 38 3.3823964220797436 7 39 4.683860862869238 7 40 4.188708634983783 7 41 3.188245445870134 7 42 2.3767877008430247 7 43 2.801590677261295 7 44 3.018794260515847 7 45 2.3863168969333692 7 46 2.7451013950439176 7 47 2.552611288568233 7 48 1.7826971884940832 7 49 1.6786003335824944 7 50 3.132276652478726 7 51 4.189042388681557 7 52 4.261908764290769 7 53 4.878750218311094 7 54 3.2313539105185036 7 55 2.725929957490493 7 56 2.0593748434718204 8 1 -0.014167183522211246 8 2 0.4858992455279463 8 3 0.8303659862444306 8 4 0.5781800396120786 8 5 0.3834408846488131 8 6 0.5995903904499542 8 7 0.8348658611586117 8 8 0.8710805131361212 8 9 0.3445259575997923 8 10 0.7155182821314554 8 11 0.27983059716382286 8 12 1.0816927601589441 8 13 1.4524635812336726 8 14 0.14728123706591845 8 15 -0.5227326753848516 8 16 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35 1.410602147171339 118 36 2.2880160524118764 118 37 2.8427272011351157 118 38 2.633302219181193 118 39 1.8396579643185156 118 40 2.064614432085232 118 41 1.469042547319411 118 42 1.420265961712591 118 43 1.8151776337830334 118 44 0.5015841155883081 118 45 0.7903024041797444 118 46 1.4679274820004409 118 47 1.8515243320464947 118 48 1.0345087826969817 118 49 1.7564988843071312 118 50 1.0589070872613355 118 51 0.3004554321598185 118 52 0.6577420976241015 118 53 -0.6312135498048477 118 54 -0.7233562344090352 118 55 0.5049154647368681 118 56 -0.08464689565861505 119 1 -0.041522806622509734 119 2 -0.22187322516246155 119 3 -0.05586935895748013 119 4 0.23645185897812127 119 5 0.14331930410170202 119 6 0.11370241823978944 119 7 0.03286846645605124 119 8 9.111688026379738E-4 119 9 -0.5082747371461949 119 10 -0.08083430145516957 119 11 -0.8174338405261494 119 12 -1.029466597201015 119 13 -1.2218742390008046 119 14 -0.9868915809974794 119 15 -1.1510435742124727 119 16 -0.2855093221051703 119 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0.6397688138380069 120 36 0.37134938574521215 120 37 1.69689386780178 120 38 1.737468885847857 120 39 0.9229912976518477 120 40 1.7521144320852322 120 41 1.9482092139860754 120 42 1.8369326283792589 120 43 0.7526776337830334 120 44 2.126584115588308 120 45 1.5403024041797444 120 46 2.467927482000441 120 47 2.7681909987131625 120 48 2.9928421160303174 120 49 3.256498884307131 120 50 2.7255737539280034 120 51 2.7796220988264864 120 52 2.0119087642907694 120 53 1.0771197835284845 120 54 0.5474770989243005 120 55 -0.32841786859646405 120 56 0.3736864376747189 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49.0 3 15.0 4 53.0 5 24.0 6 54.0 7 301.0 8 139.0 9 270.0 10 291.0 11 219.0 12 258.0 13 376.0 14 351.0 15 374.0 16 456.0 17 495.0 18 589.0 19 737.0 20 920.0 21 1090.0 22 1040.0 23 1348.0 24 1485.0 25 1295.0 26 739.0 27 663.0 28 775.0 29 891.0 30 1077.0 31 1272.0 32 1617.0 33 1877.0 34 2350.0 35 2755.0 36 3785.0 37 6121.0 38 7304.0 39 6347.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 30.86623027187663 52.83522750090358 7.0900767037468375 9.208465523472952 11 29.069113690213243 47.22501104373318 14.033572948877554 9.672302317176017 12 58.14625918637806 20.053411509577927 11.256576041122846 10.543753262921168 13 16.57764748403679 29.61929239789567 19.346612585839924 34.45644753222762 14 17.752299104453638 51.94771294325528 18.98919722099514 11.310790731295931 15 41.08268744227139 20.302397494076544 28.155495763222362 10.459419300429703 16 13.236416208184409 22.687843861692304 45.8555881289908 18.220151801132484 17 35.534717481225655 20.316453154491786 14.228344243203084 29.920485121079476 18 37.24950805188546 23.701859363077787 18.308501666599735 20.74013091843701 19 16.56158387213365 22.814344805429503 46.369623709891165 14.25444761254568 20 20.452993855668446 41.17304525922654 19.762258543833582 18.611702341271435 21 20.059435364041605 22.095498172764145 35.43431990683105 22.410746556363197 22 21.517208144251235 28.55307015782499 14.493393839604835 35.43632785831895 23 21.36187473644049 49.04716962187995 15.699110423904095 13.891845217775458 24 14.513473354483756 48.9638970322477 21.13368941006385 15.38894020320469 25 15.88302637128683 26.674566671353112 24.76048926469702 32.681917692663035 26 36.654475302439614 23.351151481049797 21.639001647843735 18.355371568666854 27 17.8758038585209 19.50361736334405 24.399115755627008 38.22146302250804 28 16.17883563846896 42.481505307172725 26.93591186876809 14.403747185590221 29 31.631873215075824 23.587144523550943 33.50026145368247 11.280720807690761 30 14.035299563283624 41.85634647507497 34.651532532351226 9.456821429290185 31 30.95257267090748 22.928577180127224 37.215154199210886 8.90369594975441 32 15.412958563202189 21.413039101195988 53.28192324729191 9.89207908830991 33 22.47779411468509 18.244073395234548 39.060202622409314 20.21792986767105 34 18.859260452751148 19.670909530331105 40.862402320148234 20.607427696769516 35 14.129427408631178 34.21042027642342 41.20763992424548 10.452512390699924 36 20.0524246395806 22.000201633229153 37.98165137614679 19.965722351043453 37 10.424476962147782 20.199943564316523 53.432498891441924 15.943080582093764 38 15.130981910581404 19.779377659467198 50.05344142618024 15.03619900377115 39 12.147496671642394 21.53548230927502 45.88493968612579 20.43208133295679 40 13.68344539342177 20.87952026167545 52.23009671492317 13.206937629979606 41 14.508766259998385 23.12151571463198 51.652258220893586 10.717459804476045 42 17.273149738346838 23.27399834320005 49.30798294708343 10.14486897136969 43 14.902903792915312 24.844908763917797 47.729706791682666 12.522480651484228 44 13.379000969932106 27.754203039120597 50.46071774975752 8.406078241189784 45 17.46776084407972 23.907506973359745 45.391518777539716 13.23321340502082 46 20.28909329829172 23.18204791266552 43.88153239664409 12.647326392398666 47 18.15754879158661 23.927596319142484 44.7062392557387 13.208615633532208 48 18.089669404766724 24.16339578359568 45.75122405211832 11.995710759519282 49 17.051269086864192 25.221963798159567 46.75498028112044 10.9717868338558 50 13.897207608255766 25.55847834884662 48.607851072440305 11.936462970457304 51 16.58744766489351 25.86112740438098 45.692846018486684 11.85857891223883 52 16.566143134715023 24.8988018134715 46.34472150259067 12.190333549222798 53 11.628848450498957 26.310143108718094 48.29058964030524 13.770418800477705 54 13.171531516942633 28.91583336707016 44.38484271891826 13.527792397068945 55 17.740334960205757 24.332205998501387 44.986735251827696 12.940723789465158 56 15.470911042261507 28.38021181378207 43.94427232043416 12.204604823522265 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 513.0 9 1026.0 10 644.0 11 262.0 12 211.0 13 164.5 14 169.0 15 363.0 16 557.0 17 744.5 18 932.0 19 912.5 20 893.0 21 980.0 22 1273.0 23 1479.0 24 1900.0 25 2321.0 26 2573.5 27 2826.0 28 3030.0 29 3160.5 30 3087.0 31 2524.5 32 1962.0 33 3342.0 34 4722.0 35 3018.5 36 1315.0 37 1062.0 38 796.0 39 783.0 40 724.0 41 665.0 42 963.0 43 1261.0 44 1509.5 45 1758.0 46 1375.0 47 966.0 48 940.0 49 1354.0 50 1768.0 51 2570.0 52 3372.0 53 3081.5 54 2452.5 55 2114.0 56 1854.5 57 1595.0 58 1410.5 59 1226.0 60 1035.5 61 845.0 62 675.5 63 538.0 64 570.0 65 621.0 66 672.0 67 645.5 68 619.0 69 467.5 70 316.0 71 215.5 72 80.0 73 45.0 74 29.5 75 14.0 76 10.0 77 6.0 78 5.5 79 3.0 80 1.0 81 1.0 82 1.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.006023854463676158 24 0.0 25 0.026103369342596685 26 0.08031805951568209 27 0.0843339624914662 28 0.11646118629773904 29 0.16063611903136418 30 0.22689851813180195 31 0.2530018874743986 32 0.2730814023533191 33 0.30721657764748406 34 0.3011927231838079 35 0.3373358499658648 36 0.4156459579936549 37 0.3774948797237059 38 0.4317095698967913 39 0.457812939239388 40 0.5521866591703144 41 0.5903377374402634 42 0.6204570097586442 43 0.6325047186859966 44 0.6304967671981045 45 0.6586080880285932 46 0.6766796514196217 47 0.7168386811774627 48 0.7549897594474118 49 0.7168386811774627 50 0.767037468374764 51 0.724870487129031 52 0.7911328862294688 53 0.8011726436689289 54 0.803180595156821 55 0.8493634793783382 56 0.84133167342677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 49802.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.55825067266375 #Duplication Level Percentage of deduplicated Percentage of total 1 78.78124873384384 39.04260873057307 2 10.45743689477736 10.365045580498775 3 4.270491471172157 6.349142604714671 4 2.0339532433856005 4.031966587687242 5 1.2074065070296989 2.991847716959158 6 0.7738746404116527 2.301112405124292 7 0.5631862566346582 1.953736797718967 8 0.29577407722539606 1.1726436689289588 9 0.3200842753535108 1.4276535078912493 >10 1.1506827113974312 9.268704068109715 >50 0.0688788946963251 2.5822256134291797 >100 0.06077549532028686 6.076061202361351 >500 0.008103399376038248 3.6825830287940247 >1k 0.008103399376038248 8.754668487209349 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 3233 6.4917071603550065 No Hit AAAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1127 2.262961326854343 No Hit AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 920 1.8473153688606883 No Hit AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 914 1.835267659933336 No Hit AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 464 0.9316894903819123 No Hit AAAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 457 0.917633829966668 No Hit AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 0.5983695433918317 No Hit AAAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 260 0.5220673868519337 No Hit AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGT 185 0.3714710252600297 No Hit AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC 181 0.3634392193084615 No Hit AAAACGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 180 0.36143126782056945 No Hit AAAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.305208626159592 No Hit AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC 146 0.2931609172322397 No Hit AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 134 0.26906549937753504 Illumina Single End Adapter 2 (95% over 23bp) AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC 127 0.2550098389622907 No Hit AAAACGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 122 0.2449700815228304 Illumina Single End Adapter 2 (96% over 30bp) AAAACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.23493032408337017 No Hit AAAACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 102 0.20481105176498934 No Hit AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC 101 0.2028031002770973 No Hit AAAACGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 100 0.20079514878920526 No Hit AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGG 94 0.18874743986185294 No Hit AAAACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.1867394883739609 No Hit AAAACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG 93 0.1867394883739609 No Hit AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT 89 0.17870768242239268 No Hit AAAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 88 0.17669973093450062 No Hit AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT 82 0.1646520220071483 No Hit AAAACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 0.15862816754347214 No Hit AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC 78 0.1566202160555801 No Hit AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA 72 0.14457250712822778 No Hit AAAACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 69 0.13854865266455163 No Hit AAAACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT 69 0.13854865266455163 Illumina Single End Adapter 2 (100% over 29bp) AAAACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.13453274968876752 No Hit AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCG 55 0.11043733183406289 No Hit AAAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.10842938034617085 No Hit AAAACGGGGTTCTCTCTTCAATTACTGGTGAATTGTTAACAGGTAGATTTGTTTTT 53 0.10642142885827878 No Hit AAAACGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTC 51 0.10240552588249467 Illumina Single End Adapter 2 (96% over 28bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.05220673868519336 0.0 0.0 0.0 0.0 11 0.11646118629773905 0.0 0.0 0.0 0.0 12 0.12047708927352316 0.0 0.0 0.0 0.0 13 0.12047708927352316 0.0 0.0 0.0 0.0 14 0.17469177944660857 0.0 0.0 0.0 0.0 15 0.17669973093450062 0.0 0.0 0.0 0.0 16 0.18071563391028472 0.0 0.0 0.0 0.0 17 0.1827235853981768 0.0 0.0 0.0 0.0 18 0.18473153688606883 0.0 0.0 0.0 0.0 19 0.18473153688606883 0.0 0.0 0.0 0.0 20 0.22288261515601784 0.0 0.0 0.0 0.0 21 0.228906469619694 0.0 0.0 0.0 0.0 22 0.2489859844986145 0.0 0.0 0.0 0.0 23 0.2730814023533191 0.0 0.0 0.0 0.0 24 0.3132404321111602 0.0 0.0 0.0 0.0 25 0.3152483835990523 0.0 0.0 0.0 0.0 26 0.32328018955062043 0.0 0.0 0.0 0.0 27 0.33131199550218865 0.0 0.0 0.0 0.0 28 0.5180514838761495 0.0 0.0 0.0 0.0 29 0.5260832898277178 0.0 0.0 0.0 0.0 30 0.6043933978555078 0.0 0.0 0.0 0.0 31 0.6144331552949681 0.0 0.0 0.0 0.0 32 0.6164411067828601 0.0 0.0 0.0 0.0 33 0.6204570097586443 0.0 0.0 0.0 0.0 34 0.6264808642223204 0.0 0.0 0.0 0.0 35 0.8132203525962812 0.0 0.0 0.0 0.0 36 0.8152283040841733 0.0 0.0 0.0 0.0 37 0.8172362555720654 0.0 0.0 0.0 0.0 38 0.8192442070599575 0.0 0.0 0.0 0.0 39 0.8212521585478495 0.0 0.0 0.0 0.0 40 0.8332998674752018 0.0 0.0 0.0 0.0 41 0.8373157704509859 0.0 0.0 0.0 0.0 42 0.8453475764025541 0.0 0.0 0.0 0.0 43 0.8533793823541224 0.0 0.0 0.0 0.0 44 0.8654270912814747 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCAAT 25 1.9639268E-5 50.397762 49 TTGTATG 15 0.008124884 50.397762 50 TGGTATG 20 3.9857064E-4 50.397762 50 GCCGCTC 20 3.9857064E-4 50.397762 50 TGGCGCT 15 0.008124884 50.397762 47 TTTGTAT 15 0.008124884 50.397762 49 GTTGCAA 25 1.9639268E-5 50.397762 48 CATCGAA 50 5.456968E-12 50.397762 50 TCGTTGC 25 1.9758048E-5 50.346546 46 GATTTGT 15 0.008157653 50.346546 46 GCATTTT 25 1.9877421E-5 50.295433 45 AGATTTG 15 0.008190519 50.295433 45 TCTCGTT 25 1.9997393E-5 50.244423 44 GTCTCGT 25 1.9997393E-5 50.244423 43 AGCCGTC 25 1.9997393E-5 50.244423 39 CGTCTCG 25 1.9997393E-5 50.244423 42 GTAGATT 15 0.008223484 50.24442 43 ACGTAGA 15 0.008223484 50.24442 41 CGTAGAG 15 0.008223484 50.24442 42 GAGCATT 15 0.008223484 50.24442 43 >>END_MODULE