FastQCFastQC Report
Tue 31 May 2016
SRR212483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31473
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5921.8809773456613605No Hit
CTCCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4621.4679248879992375No Hit
CTCCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4461.4170876624408224No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG3931.248689352778572No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3881.2328027197915674No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3871.2296253931941665No Hit
CTCCATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT2830.899183427064468No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2470.7847996695580339No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2090.6640612588567979No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1610.5115495821815524No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.49248562259714673No Hit
CTCCATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1530.48613096940234485No Hit
CTCCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1420.4511803768309344No Hit
CTCCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1420.4511803768309344No Hit
CTCCATGGGACTGCGAGGCTGTGGTATCCAGTCTCCTGGGGAGCCCCCTGCCTTTTGA1290.4098751310647222No Hit
CTCCATGGGACCTTCAAACCTGGTGCGTTCATTCCTGGAGTTCCTGTCCAGCCCGTGG1150.36539255870110887No Hit
CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1070.33997394592190133No Hit
CTCCATGGGAACTAAGTTCTTAATATTTACTAGAGACAGGAAGATCACTGTTGCTGAG990.31455533314269374No Hit
CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT980.3113780065452928No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC860.27325008737648143No Hit
CTCCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT820.2605407809868776No Hit
CTCCATGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC780.24783147459727387No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG770.24465414799987292No Hit
CTCCATGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG730.23194484161026913No Hit
CTCCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT710.2255901884154672No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC670.21288088202586342No Hit
CTCCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA650.20652622883106153No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA600.19063959584405682No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC590.18746226924665585No Hit
CTCCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA580.1842849426492549No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC580.1842849426492549No Hit
CTCCATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA550.17475296285705208No Hit
CTCCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT510.16204365646744828No Hit
CTCCATGGGCTTTCTTTAAGCTAAGCTGTATTTTTAGTGAGCTGCCCTTTTAAAAAGG510.16204365646744828No Hit
CTCCATGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG510.16204365646744828No Hit
CTCCATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT500.15886632987004734No Hit
CTCCATGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG500.15886632987004734No Hit
CTCCATGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCT490.1556890032726464No Hit
CTCCATGGGGGCCCTTTTGCTGCCTTGAGCTTTGGACCTAGGCTCCGCTCTTACCAGT490.1556890032726464No Hit
CTCCATGGGAGTCATTAACATTCTACAACAGAAGAGCCTCCCTTTATCCACTTACGAA480.15251167667524546No Hit
CTCCATGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC480.15251167667524546No Hit
CTCCATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT460.14615702348044357No Hit
CTCCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC460.14615702348044357No Hit
CTCCATGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG450.14297969688304263No Hit
CTCCATGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTC440.13980237028564166No Hit
CTCCATGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAG430.13662504368824072No Hit
CTCCATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG430.13662504368824072No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA410.1302703904934388No Hit
CTCCATGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG410.1302703904934388No Hit
CTCCATGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG410.1302703904934388No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT410.1302703904934388No Hit
CTCCATGGGAAGAATGTAACTGATAGCATATTCTCTGTTGTACCAAAATTACTCTCTT390.12391573729863693No Hit
CTCCATGGGAAGAGGAGAAGAACTAGTTACGGGGGTTTCAGAGCAAAGGGGGGCTCTA380.12073841070123599No Hit
CTCCATGGGACCTAAAACAGTAGACTCCCTGCATCTGACATTGGCCTGCCTGCCCCAT380.12073841070123599No Hit
CTCCATGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTGAG380.12073841070123599No Hit
CTCCATGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT370.11756108410383503No Hit
CTCCATGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA370.11756108410383503No Hit
CTCCATGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT370.11756108410383503No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC370.11756108410383503No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC370.11756108410383503No Hit
CTCCATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG360.11438375750643409No Hit
CTCCATGGGAGTGGCTCTCAATGACCCTTTCAGAGGTTGCCCTAAGCTATCAGACAAT360.11438375750643409No Hit
CTCCATGGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGACCCG360.11438375750643409No Hit
CTCCATGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT360.11438375750643409No Hit
CTCCATGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG360.11438375750643409No Hit
CTCCATGGGGCGCCACAGTCCTCTACCCCTGCGTGGTGTATGACCGTGGGCCCGAGAG350.11120643090903314No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGCAGATAAGAGCACCCGACTGTTCTTCTGA350.11120643090903314No Hit
CTCCATGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG350.11120643090903314No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC340.10802910431163218No Hit
CTCCATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG340.10802910431163218No Hit
CTCCATGGGGCTTTTCCGATCCTGTACTCCAGGGACAAAACTGGGTGGAGGTCCTCTT330.10485177771423124No Hit
CTCCATGGGATCTCAAAACTGCTTTTACAAAAGCAGAATAGAAATGAAGTGAAAATGC330.10485177771423124No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA330.10485177771423124No Hit
CTCCATGGGTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGC330.10485177771423124No Hit
CTCCATGGGGCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCG320.1016744511168303No Hit
CTCCATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1016744511168303No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTTCA320.1016744511168303No Hit
CTCCATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA320.1016744511168303No Hit
CTCCATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA320.1016744511168303No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCG320.1016744511168303No Hit
CTCCATGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC320.1016744511168303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAG203.371684E-452.07643552
CTTTTGA307.668714E-752.0764352
TCGAACT307.668714E-752.0764352
TGCGAGC150.00711381552.0764349
GAGCTTA150.00711381552.0764352
ATTAAGC150.00711381552.0764351
CGAGCTT150.00711381552.0764351
GATTAAG150.00711381552.0764350
AGATTAA150.00711381552.0764349
GCGAGCT150.00711381552.0764350
TATGGGT150.00711381552.0764352
ATGGGTA353.7272912E-851.9936455
ATGGGTG600.051.993645
CTCTGGC150.00715881951.9936418
ATCCTGC150.00715881951.9936420
ATCCCCA458.731149E-1151.9936424
GTTGATC150.00715881951.9936416
ATGGGCT1950.051.993645
ATGGGCG150.00715881951.993645
CAGTCTC307.753788E-751.9936429
Produced by FastQC (version 0.11.5)