FastQCFastQC Report
Tue 31 May 2016
SRR212482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33218
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6271.8875308567644047No Hit
CTCCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4911.4781142753928593No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG4231.2734059847070867No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4211.2673851526280933No Hit
CTCCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4121.2402914082726233No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3991.2011559997591668No Hit
CTCCATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT3170.9543018845204407No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2530.7616352579926546No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2350.7074477692817147No Hit
CTCCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1560.4696249021614787No Hit
CTCCATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1540.4636040700824854No Hit
CTCCATGGGACTGCGAGGCTGTGGTATCCAGTCTCCTGGGGAGCCCCCTGCCTTTTGA1430.43048949364802214No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.4214582455295322No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1300.39135408513456565No Hit
CTCCATGGGAACTAAGTTCTTAATATTTACTAGAGACAGGAAGATCACTGTTGCTGAG1240.3732915888975856No Hit
CTCCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1230.370281172858089No Hit
CTCCATGGGACCTTCAAACCTGGTGCGTTCATTCCTGGAGTTCCTGTCCAGCCCGTGG1170.352218676621109No Hit
CTCCATGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1100.33114576434463244No Hit
CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1100.33114576434463244No Hit
CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT870.2619061954362093No Hit
CTCCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT830.24986453127822267No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG800.24083328315973265No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC730.21976037088325606No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC710.21373953880426275No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC690.20771870672526943No Hit
CTCCATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA690.20771870672526943No Hit
CTCCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA670.2016978746462761No Hit
CTCCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT670.2016978746462761No Hit
CTCCATGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG660.19868745860677944No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC650.19567704256728283No Hit
CTCCATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG610.18363537840929617No Hit
CTCCATGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG610.18363537840929617No Hit
CTCCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC600.18062496236979952No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA580.1746041302908062No Hit
CTCCATGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC540.16256246613281955No Hit
CTCCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT520.15654163405382623No Hit
CTCCATGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTC520.15654163405382623No Hit
CTCCATGGGAGTCATTAACATTCTACAACAGAAGAGCCTCCCTTTATCCACTTACGAA510.15353121801432956No Hit
CTCCATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT480.1444999698958396No Hit
CTCCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA480.1444999698958396No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCG460.1384791378168463No Hit
CTCCATGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG460.1384791378168463No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC460.1384791378168463No Hit
CTCCATGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG450.13546872177734964No Hit
CTCCATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.13245830573785297No Hit
CTCCATGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA430.1294478896983563No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGCAGATAAGAGCACCCGACTGTTCTTCTG430.1294478896983563No Hit
CTCCATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT420.12643747365885966No Hit
CTCCATGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT420.12643747365885966No Hit
CTCCATGGGAAGAGGAGAAGAACTAGTTACGGGGGTTTCAGAGCAAAGGGGGGCTCTA410.12342705761936298No Hit
CTCCATGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCT410.12342705761936298No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA410.12342705761936298No Hit
CTCCATGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTGAG410.12342705761936298No Hit
CTCCATGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAG400.12041664157986633No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC400.12041664157986633No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA390.11740622554036967No Hit
CTCCATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG390.11740622554036967No Hit
CTCCATGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG380.11439580950087302No Hit
CTCCATGGGGCTCTTTTACCGAGACCCGCCAACATGGGCCGCGTTCGCACCAAGACCG380.11439580950087302No Hit
CTCCATGGGTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGC370.11138539346137637No Hit
CTCCATGGGACCTAAAACAGTAGACTCCCTGCATCTGACATTGGCCTGCCTGCCCCAT360.10837497742187971No Hit
CTCCATGGGGCGCCACAGTCCTCTACCCCTGCGTGGTGTATGACCGTGGGCCCGAGAG360.10837497742187971No Hit
CTCCATGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCTT360.10837497742187971No Hit
CTCCATGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC360.10837497742187971No Hit
CTCCATGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG360.10837497742187971No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA360.10837497742187971No Hit
CTCCATGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA350.10536456138238305No Hit
CTCCATGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG350.10536456138238305No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTTCA340.1023541453428864No Hit
CTCCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG340.1023541453428864No Hit
CTCCATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG340.1023541453428864No Hit
CTCCATGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT340.1023541453428864No Hit
CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.1023541453428864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGT150.007106283752.09667650
TTTTACG150.007106283752.09667650
TGCCTTT150.007106283752.09667649
CAGACAA150.007106283752.09667651
TTTACGG150.007106283752.09667651
TCCGAAG150.007106283752.09667652
TTACGGC150.007106283752.09667652
TATGGAT150.007106283752.09667652
GCCCGCA203.3679005E-452.09667252
TAGGCCC203.3679005E-452.09667249
GAGAAAC203.393165E-452.018117
AAACCCC203.393165E-452.018126
CACATGT203.393165E-452.018139
TCTAGGC203.393165E-452.018147
GAAACCC203.393165E-452.018125
TGTTTCT203.393165E-452.018143
CTAGGCC203.393165E-452.018148
AGAAACC203.393165E-452.018124
GTCCAGC203.393165E-452.018146
ATGTTTC203.393165E-452.018142
Produced by FastQC (version 0.11.5)