FastQCFastQC Report
Tue 31 May 2016
SRR212481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34191
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6241.8250416776344653No Hit
CTCCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4841.4155771986780146No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG4051.1845222426954463No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3651.0675323915650319No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3601.0529086601737299No Hit
CTCCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG3601.0529086601737299No Hit
CTCCATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT2960.8657248983650669No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2650.7750577637389957No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2170.6346699423824983No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.4533356731303559No Hit
CTCCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1340.39191600128688836No Hit
CTCCATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1330.388991255008628No Hit
CTCCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1230.35974379222602443No Hit
CTCCATGGGACTGCGAGGCTGTGGTATCCAGTCTCCTGGGGAGCCCCCTGCCTTTTGA1230.35974379222602443No Hit
CTCCATGGGACCTTCAAACCTGGTGCGTTCATTCCTGGAGTTCCTGTCCAGCCCGTGG1170.34219531455646224No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1160.33927056827820185No Hit
CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1140.33342107572168117No Hit
CTCCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1010.29539937410429645No Hit
CTCCATGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC990.2895498815477757No Hit
CTCCATGGGAACTAAGTTCTTAATATTTACTAGAGACAGGAAGATCACTGTTGCTGAG960.28077564271299466No Hit
CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT920.2690766575999532No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG870.2544529262086514No Hit
CTCCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT720.21058173203474598No Hit
CTCCATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA720.21058173203474598No Hit
CTCCATGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC620.18133426925214238No Hit
CTCCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA610.17840952297388202No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC600.17548477669562165No Hit
CTCCATGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG600.17548477669562165No Hit
CTCCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC600.17548477669562165No Hit
CTCCATGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAG590.17256003041736132No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC580.16963528413910092No Hit
CTCCATGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT560.16378579158258022No Hit
CTCCATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT540.1579362990260595No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC540.1579362990260595No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA530.15501155274779913No Hit
CTCCATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG510.1491620601912784No Hit
CTCCATGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTC490.1433125676347577No Hit
CTCCATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT480.14038782135649733No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC470.13746307507823696No Hit
CTCCATGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG450.13161358252171623No Hit
CTCCATGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAG440.1286888362434559No Hit
CTCCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA430.1257640899651955No Hit
CTCCATGGGAAGAGGAGAAGAACTAGTTACGGGGGTTTCAGAGCAAAGGGGGGCTCTA420.12283934368693517No Hit
CTCCATGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC420.12283934368693517No Hit
CTCCATGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG420.12283934368693517No Hit
CTCCATGGGAGTCATTAACATTCTACAACAGAAGAGCCTCCCTTTATCCACTTACGAA410.11991459740867479No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTTCA400.11698985113041444No Hit
CTCCATGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA390.11406510485215408No Hit
CTCCATGGGATCCAGAGAGGTAATGACTTCTGGGACTTGGTAGTTACTTTGTGTGGAG390.11406510485215408No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCCAA390.11406510485215408No Hit
CTCCATGGGGAACCTCAGTGCAATATGCCCTCCCCGTGGGGTCCCTTGGCACGGTACT380.11114035857389372No Hit
CTCCATGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA380.11114035857389372No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT380.11114035857389372No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC380.11114035857389372No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA370.10821561229563337No Hit
CTCCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT370.10821561229563337No Hit
CTCCATGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA370.10821561229563337No Hit
CTCCATGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCT370.10821561229563337No Hit
CTCCATGGGAGTGGCTCTCAATGACCCTTTCAGAGGTTGCCCTAAGCTATCAGACAAT370.10821561229563337No Hit
CTCCATGGGACCTAAAACAGTAGACTCCCTGCATCTGACATTGGCCTGCCTGCCCCAT360.10529086601737299No Hit
CTCCATGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG360.10529086601737299No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC360.10529086601737299No Hit
CTCCATGGGGGGCTGCGCCTGGTCTGGCAGGCACCATGTGTGCATCCCGCGATGATTG350.10236611973911264No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC350.10236611973911264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCC150.006971814752.3515558
GGGTCCT150.006971814752.3515558
GGACTTC150.006971814752.3515558
GATGTTT150.006971814752.3515559
GGATCTC150.006971814752.3515558
GATGCCA150.006971814752.3515559
GGACATG150.006971814752.3515558
GGATCAC150.006971814752.3515558
TGGGGAC307.412327E-752.3515556
GGGATTA150.006971814752.3515557
GATTTAA150.006971814752.3515559
GACTGCG150.006971814752.3515559
GGGATGA150.006971814752.3515557
GGGATCC150.006971814752.3515557
TGGGTAT150.006971814752.3515556
TGGGTAC150.006971814752.3515556
ATGGGTG401.6989361E-952.351555
GGATGGT203.2887966E-452.351558
ATGGGAG1350.052.351555
TGGGGCG353.541936E-852.351556
Produced by FastQC (version 0.11.5)