FastQCFastQC Report
Tue 31 May 2016
SRR212480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68825
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT17362.5223392662549946No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC13221.920813657827824No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC11841.7203051216854341No Hit
CTCCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA6880.9996367598982928No Hit
CTCCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA6510.945877224845623No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5730.8325463131129677No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3900.5666545586632764No Hit
CTCCATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3860.5608427170359608No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAG3750.5448601525608427No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3440.4998183799491464No Hit
CTCCATGGGAATCAAAGATGATTTTTTTCAAGCCAATAATTTCCAAAGGAACTACT3350.4867417362876862No Hit
CTCCATGGGAGGTGACTTAATGTATCTTAATAACTACTATAGGAACAAAGGAAGTG2690.3908463494369779No Hit
CTCCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2240.32546313112967673No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.3123864874682165No Hit
CTCCATGGGACCTTCAAACCTGGTGCGTTCATTCCTGGAGTTCCTGTCCAGCCCGT2130.30948056665455864No Hit
CTCCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC2020.2934980021794406No Hit
CTCCATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2010.2920450417726117No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1920.2789683981111515No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1900.2760624772974936No Hit
CTCCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG1860.270250635670178No Hit
CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1840.26734471485652017No Hit
CTCCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1700.24700326916091536No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1640.2382855067199419No Hit
CTCCATGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1560.22666182346531058No Hit
CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1510.219397021431166No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1500.2179440610243371No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1430.20777333817653468No Hit
CTCCATGGGACTGCGAGGCTGTGGTATCCAGTCTCCTGGGGAGCCCCCTGCCTTTT1420.20632037776970577No Hit
CTCCATGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAA1360.1976026153287323No Hit
CTCCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1360.1976026153287323No Hit
CTCCATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1330.19324373410824555No Hit
CTCCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1320.19179077370141664No Hit
CTCCATGGGAACTAAGTTCTTAATATTTACTAGAGACAGGAAGATCACTGTTGCTG1280.185978932074101No Hit
CTCCATGGGGCTCTCCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCC1260.18307301126044315No Hit
CTCCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1220.1772611696331275No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1220.1772611696331275No Hit
CTCCATGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1190.17290228841264074No Hit
CTCCATGGGATAGCTTATTCTGAAACCAAATCTGATTGACTGTGGCCCATGAACAC1190.17290228841264074No Hit
CTCCATGGGAGTCATTAACATTCTACAACAGAAGAGCCTCCCTTTATCCACTTACG1170.16999636759898293No Hit
CTCCATGGGAAGAGGAGAAGAACTAGTTACGGGGGTTTCAGAGCAAAGGGGGGCTC1150.1670904467853251No Hit
CTCCATGGGAGTTGAAGTTTCCTCTTCTCATGTAATTCTAACTTGTGTTGACTTGA1110.16127860515800946No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCC1070.1554667635306938No Hit
CTCCATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA1050.15256084271703596No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAGCAGT1020.1482019614965492No Hit
CTCCATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT990.1438430802760625No Hit
CTCCATGGGACGTTTTTTCTACTTTCCTTATGAAAATAAAGTAGTTGCTTTGTTCT940.1365782782419179No Hit
CTCCATGGGAGTGGCTCTCAATGACCCTTTCAGAGGTTGCCCTAAGCTATCAGACA930.135125317835089No Hit
CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.13221939702143115No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTT890.12931347620777334No Hit
CTCCATGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT880.12786051580094443No Hit
CTCCATGGGGCTCTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTT870.1264075553941155No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTC870.1264075553941155No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG860.1249545949872866Illumina Single End Adapter 2 (95% over 23bp)
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAGCAGG860.1249545949872866No Hit
CTCCATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCTGCTCTTCC810.11768979295314203No Hit
CTCCATGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC790.11478387213948421No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC770.11187795132582637No Hit
CTCCATGGGAGCACTGGGCATTTCCCTCCAGGTAGTATTCTTGCTGCTCTGATTCC770.11187795132582637No Hit
CTCCATGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG770.11187795132582637No Hit
CTCCATGGGATGCAAGAGGGGCGAATGGGCATTTAGTGGGTCCCCAGAAGTGCCAT750.10897203051216856No Hit
CTCCATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGA730.10606610969851071No Hit
CTCCATGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC720.1046131492916818No Hit
CTCCATGGGATTTAGAGCAATTAAAACTGCCATTACAGTAGTTTCAAGAGTGCTCT710.10316018888485289No Hit
CTCCATGGGGAACCTCAGTGCAATATGCCCTCCCCGTGGGGTCCCTTGGCACGGTA710.10316018888485289No Hit
CTCCATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA700.10170722847802396No Hit
CTCCATGGGAGTTCGAACGACACAGCCTCACAATCAACATCTCCTTCCCAGCCACG700.10170722847802396No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGCAGATAAGAGCACCCGACTGTTCTTC690.10025426807119504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCT251.9890664E-550.32452449
GCGACTG251.9890664E-550.32452450
CCCGGAG204.025445E-450.32452449
GAACTAC550.050.32452449
CCTTAAC204.025445E-450.32452448
GCCCGGA204.025445E-450.32452448
GTTGCTG204.025445E-450.32452450
ATTTAAT204.025445E-450.32452449
CTACACG150.00818520450.3245247
ACACGAC150.00818520450.3245249
TGGCACG150.00818520450.3245247
GGCACGG150.00818520450.3245248
AAACAAC150.00818520450.3245249
GTCCGCG150.00818520450.3245249
CCACTTA150.00818520450.3245248
TTTAATA150.00818520450.3245250
CACGGTA150.00818520450.3245250
CAGCTGA150.00818520450.3245250
CGGCGTC251.9977364E-550.287645
ATGCCCG251.9977364E-550.287646
Produced by FastQC (version 0.11.5)