FastQCFastQC Report
Tue 31 May 2016
SRR212479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66693
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT16522.4770215764772914No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC15012.2506110086515827No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC12511.87575907516531No Hit
CTCCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA6610.9911085121377056No Hit
CTCCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA6370.9551227265230234No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG6040.9056422713028354No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4670.7002234117523578No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAG4380.6567405874679502No Hit
CTCCATGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA4280.6417465101284993No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3430.5142968527431665No Hit
CTCCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC3220.4828092903303195No Hit
CTCCATGGGAATCAAAGATGATTTTTTTCAAGCCAATAATTTCCAAAGGAACTACT2770.4153359423027904No Hit
CTCCATGGGAGGTGACTTAATGTATCTTAATAACTACTATAGGAACAAAGGAAGTG2710.4063394958991199No Hit
CTCCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2490.37335252575232786No Hit
CTCCATGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2460.36885430255049256No Hit
CTCCATGGGGCTCTCCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCC2270.34036555560553583No Hit
CTCCATGGGACCTTCAAACCTGGTGCGTTCATTCCTGGAGTTCCTGTCCAGCCCGT2140.32087325506424963No Hit
CTCCATGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2060.3088779931926889No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.3088779931926889No Hit
CTCCATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2050.3073785854587438No Hit
CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1880.28188865398167723No Hit
CTCCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG1830.2743916153119518No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1740.26089694570644595No Hit
CTCCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1730.25939753797250087No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1640.24590286836699504No Hit
CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1640.24590286836699504No Hit
CTCCATGGGACTGCGAGGCTGTGGTATCCAGTCTCCTGGGGAGCCCCCTGCCTTTT1610.24140464516515975No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1510.22641056782570884No Hit
CTCCATGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1490.22341175235781868No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1460.21891352915598342No Hit
CTCCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1440.2159147136880932No Hit
CTCCATGGGACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAA1430.21441530595414812No Hit
CTCCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1410.21141649048625794No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1400.20991708275231283No Hit
CTCCATGGGAGCACTGGGCATTTCCCTCCAGGTAGTATTCTTGCTGCTCTGATTCC1270.19042478221102666No Hit
CTCCATGGGAACTAAGTTCTTAATATTTACTAGAGACAGGAAGATCACTGTTGCTG1260.18892537447708155No Hit
CTCCATGGGAAGAGGAGAAGAACTAGTTACGGGGGTTTCAGAGCAAAGGGGGGCTC1150.17243188940368553No Hit
CTCCATGGGATAGCTTATTCTGAAACCAAATCTGATTGACTGTGGCCCATGAACAC1140.17093248166974045No Hit
CTCCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1130.16943307393579535No Hit
CTCCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1110.1664342584679052No Hit
CTCCATGGGAGTCATTAACATTCTACAACAGAAGAGCCTCCCTTTATCCACTTACG1030.15443899659634444No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCC970.1454425501926739No Hit
CTCCATGGGATGCAAGAGGGGCGAATGGGCATTTAGTGGGTCCCCAGAAGTGCCAT960.1439431424587288No Hit
CTCCATGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC940.14094432699083861No Hit
CTCCATGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC920.13794551152294843No Hit
CTCCATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA910.13644610378900335No Hit
CTCCATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTT880.13194788058716805No Hit
CTCCATGGGGCTCTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTT860.1289490651192779No Hit
CTCCATGGGAGTTGAAGTTTCCTCTTCTCATGTAATTCTAACTTGTGTTGACTTGA830.12445084191744261No Hit
CTCCATGGGAGTGGCTCTCAATGACCCTTTCAGAGGTTGCCCTAAGCTATCAGACA830.12445084191744261No Hit
CTCCATGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC800.11995261871560735No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT800.11995261871560735No Hit
CTCCATGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT800.11995261871560735No Hit
CTCCATGGGGCGCCACAGTCCTCTACCCCTGCGTGGTGTATGACCGTGGGCCCGAG790.11845321098166224No Hit
CTCCATGGGACGTTTTTTCTACTTTCCTTATGAAAATAAAGTAGTTGCTTTGTTCT790.11845321098166224No Hit
CTCCATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCTGCTCTTCC780.11695380324771715No Hit
CTCCATGGGACCTACAGGGTTACAGATCTCTTTAGCTCCTTGGGTAATATCTCTAG750.11245558004588188No Hit
CTCCATGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC730.10945676457799171No Hit
CTCCATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA720.1079573568440466No Hit
CTCCATGGGAGTTCGAACGACACAGCCTCACAATCAACATCTCCTTCCCAGCCACG720.1079573568440466No Hit
CTCCATGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG720.1079573568440466No Hit
CTCCATGGGGAACCTCAGTGCAATATGCCCTCCCCGTGGGGTCCCTTGGCACGGTA720.1079573568440466No Hit
CTCCATGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA710.10645794911010151No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG710.10645794911010151Illumina Single End Adapter 2 (95% over 23bp)
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC710.10645794911010151No Hit
CTCCATGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG710.10645794911010151No Hit
CTCCATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTT700.10495854137615641No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTCTTC700.10495854137615641No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC690.10345913364221133No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAGCAGT690.10345913364221133No Hit
CTCCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG690.10345913364221133No Hit
CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10046031817432113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGT150.00830969550.1312245
ACAAAGG600.050.1312245
CAAGACC150.00830969550.1312239
CTTTACT150.00830969550.1312236
GCAAGCA150.00830969550.1312249
AGGATTT150.00830969550.1312235
ACTTGAT150.00830969550.1312247
GCGACAG150.00830969550.1312244
AATTTCC700.050.1312238
AGGACCT150.00830969550.1312235
CCTAAGG150.00830969550.1312239
TCGGTAT150.00830969550.1312248
TGCGACA150.00830969550.1312243
ATGGTCC150.00830969550.1312250
CTAAGGT150.00830969550.1312240
AGGAAGT550.050.1312249
GAGCTCC150.00830969550.1312238
TTTTACG150.00830969550.1312249
GTCCCCA150.00830969550.1312240
AAGACCT150.00830969550.1312240
Produced by FastQC (version 0.11.5)