FastQCFastQC Report
Tue 31 May 2016
SRR212478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99407
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC26542.6698321043789672No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC20352.0471395374571206No Hit
TTTAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA17521.7624513364250003No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC17351.7453499250555795No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA16751.6849920025752714No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12541.2614805798384419No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12401.2473970645930368No Hit
TTTAGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC10051.0109952015451629No Hit
TTTAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG8580.8631182914684076No Hit
TTTAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG7760.7806291307453197No Hit
TTTAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA7100.7142354160169807No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3930.3953443922460189No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3530.3551057772591467No Hit
TTTAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3510.35309384650980313No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3490.35108191576045955No Hit
TTTAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC3460.3480640196364441No Hit
TTTAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3340.33599243514038246No Hit
TTTAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3330.33498646976571067No Hit
TTTAGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3290.33096260826702345No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3130.31486716227227457No Hit
TTTAGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3040.30581347390022834No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2880.2897180279054795No Hit
TTTAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2550.25652117054130996No Hit
TTTAGGGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG2550.25652117054130996No Hit
TTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2450.2464615167945919No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2440.24545555141992012No Hit
TTTAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2390.24042572454656108No Hit
TTTAGGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC2180.21930045167845325No Hit
TTTAGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2110.2122586940557506No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.20622290180771977No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC2030.20421097105837618No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCAGGGTAGAGAGGAGCGTGGCCTTCTC2000.20119307493436075No Hit
TTTAGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2000.20119307493436075No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2000.20119307493436075No Hit
TTTAGGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC1950.19616324806100174No Hit
TTTAGGGGGAGTTAAAAAAATCCATGAGAACGTTTTCTAGGATTTCCATTTTCATT1870.1881155250636273No Hit
TTTAGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1860.1871095596889555No Hit
TTTAGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1800.18107376744092468No Hit
TTTAGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1800.18107376744092468No Hit
TTTAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC1750.17604394056756567No Hit
TTTAGGGGGTGATCAGAAGTTCACCAGCATCCTTAGCTGCCGAGCAAGGATGAGGC1740.17503797519289385No Hit
TTTAGGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA1730.17403200981822206No Hit
TTTAGGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT1710.17202007906887845No Hit
TTTAGGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA1690.17000814831953484No Hit
TTTAGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1590.15994849457281682No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1560.1569305984488014No Hit
TTTAGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAA1540.1549186676994578No Hit
TTTAGGGGGGCTTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1530.153912702324786No Hit
TTTAGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1520.15290673695011417No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACA1480.14888287545142695No Hit
TTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1400.14083515245405254No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1400.14083515245405254No Hit
TTTAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACC1360.13681129095536532No Hit
TTTAGGGGGGAGAGGATCAAGCTGCTTTTGCCTCCCAAGCGCTGGGATTTAAGGAG1300.1307754987073345No Hit
TTTAGGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1290.1297695333326627No Hit
TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.12675163720864727No Hit
TTTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1250.12574567183397548No Hit
TTTAGGGGGATTCTTACATCTTTTCCTTGCCCTCTGTGCTTTGAAAGCACTGAATA1240.12473970645930368No Hit
TTTAGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT1210.12172181033528826No Hit
TTTAGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTA1210.12172181033528826No Hit
TTTAGGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1210.12172181033528826No Hit
TTTAGGGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC1210.12172181033528826No Hit
TTTAGGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAA1200.12071584496061646No Hit
TTTAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGG1180.11870391421127284No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1150.11568601808725743No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA1140.11468005271258563No Hit
TTTAGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1140.11468005271258563No Hit
TTTAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGA1130.11367408733791383No Hit
TTTAGGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1100.11065619121389841No Hit
TTTAGGGGGGCTCTTGTTAAAGAAAGGGTAGCCTCGAGTGAGACAAAATCGCAAAC1070.107638295089883No Hit
TTTAGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCC1070.107638295089883No Hit
TTTAGGGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTA1060.10663232971521121No Hit
TTTAGGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTG1050.1056263643405394No Hit
TTTAGGGGGATGCCTGCTGGGGAAATGCAAAGCAGAGGTAGAAGTGTAGCAGCCAC1040.10462039896586758No Hit
TTTAGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG1030.10361443359119579No Hit
TTTAGGGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGA1020.10260846821652399No Hit
TTTAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGC1020.10260846821652399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACAT251.9911655E-550.343550
CTTTCAA204.0267783E-450.34349449
TGTTGAC204.0267783E-450.34349448
CATTGGC150.00818377850.34349449
TGGTATG309.90176E-750.34349450
TGCAATG150.00818377850.34349450
CGCTCTT150.00818377850.34349448
GCGAATT150.00818377850.34349448
AGGAACT150.00818377850.34349448
ACATTGG150.00818377850.34349448
GAACTTG150.00818377850.34349450
CGAATTC150.00818377850.34349449
CTAGCGA204.0267783E-450.34349449
TTGGTAT354.9491064E-850.34349449
GATTAAG204.0267783E-450.34349450
AGCAATC150.00818377850.34349450
GGAACTT150.00818377850.34349449
CCAAGAC150.00818377850.34349450
AGATTAA204.0267783E-450.34349449
TCCCCTA204.0267783E-450.34349450
Produced by FastQC (version 0.11.5)