FastQCFastQC Report
Tue 31 May 2016
SRR212477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94314
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC28042.9730474796954853No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC19552.0728629895879718No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC17741.8809508662552747No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA16371.7356914137879849No Hit
TTTAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA15791.6741947112835844No Hit
TTTAGGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC13991.4833428759251013No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11781.2490192336238521No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC11521.2214517462942935No Hit
TTTAGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG9310.9871281039930445No Hit
TTTAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG7970.8450495154483958No Hit
TTTAGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA6830.724176686388023No Hit
TTTAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT5020.5322645630553258No Hit
TTTAGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4050.4294166295565876No Hit
TTTAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC3960.4198740377886634No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3810.40396971817545646No Hit
TTTAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3530.3742816548974702No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3360.3562567593358356No Hit
TTTAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3270.34671416756791146No Hit
TTTAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3200.33929215174841487No Hit
TTTAGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3030.3212672561867803No Hit
TTTAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2780.2947600568314354No Hit
TTTAGGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2670.2830968891150836No Hit
TTTAGGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2540.26931314545030427No Hit
TTTAGGGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG2460.2608308416565939No Hit
TTTAGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2400.25446911381131115No Hit
TTTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2380.2523485378628836No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2360.25022796191445595No Hit
TTTAGGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC2300.2438662340691732No Hit
TTTAGGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2230.2364442182496766No Hit
TTTAGGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1990.2109973068685455No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.2109973068685455No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC1980.2099370188943317No Hit
TTTAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC1910.2025150030748351No Hit
TTTAGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1800.19085183535848335No Hit
TTTAGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1760.18661068346162818No Hit
TTTAGGGGGGCCTTTTCCTGTGGGAGCAGCAGGGTAGAGAGGAGCGTGGCCTTCTC1720.18236953156477298No Hit
TTTAGGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT1720.18236953156477298No Hit
TTTAGGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA1680.1781283796679178No Hit
TTTAGGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC1670.17706809169370402No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1640.17388722777106264No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.17176665182263504No Hit
TTTAGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAA1610.17070636384842122No Hit
TTTAGGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1600.16964607587420744No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1450.1537417562610005No Hit
TTTAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACC1390.1473800284157177No Hit
TTTAGGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1350.1431388765188625No Hit
TTTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1340.14207858854464872No Hit
TTTAGGGGGGCTTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1310.13889772462200733No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACA1280.13571686069936595No Hit
TTTAGGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1260.13359628475093838No Hit
TTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1230.13041542082829696No Hit
TTTAGGGGGTGATCAGAAGTTCACCAGCATCCTTAGCTGCCGAGCAAGGATGAGGC1230.13041542082829696No Hit
TTTAGGGGGAGTTAAAAAAATCCATGAGAACGTTTTCTAGGATTTCCATTTTCATT1220.12935513285408318No Hit
TTTAGGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA1210.12829484487986936No Hit
TTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1210.12829484487986936No Hit
TTTAGGGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1180.12511398095722798No Hit
TTTAGGGGGGAGAGGATCAAGCTGCTTTTGCCTCCCAAGCGCTGGGATTTAAGGAG1170.12405369298301419No Hit
TTTAGGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGA1120.1187522531119452No Hit
TTTAGGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA1110.1176919651377314No Hit
TTTAGGGGGATTCTTACATCTTTTCCTTGCCCTCTGTGCTTTGAAAGCACTGAATA1110.1176919651377314No Hit
TTTAGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1090.11557138918930382No Hit
TTTAGGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGT1070.11345081324087622No Hit
TTTAGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTA1070.11345081324087622No Hit
TTTAGGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1050.11133023729244862No Hit
TTTAGGGGGGCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCAC1040.11026994931823485No Hit
TTTAGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG1040.11026994931823485No Hit
TTTAGGGGGGGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCT1030.10920966134402103No Hit
TTTAGGGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGA1020.10814937336980723No Hit
TTTAGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA1020.10814937336980723No Hit
TTTAGGGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGGTCTGGGA1020.10814937336980723No Hit
TTTAGGGGGGCTCTTGTTAAAGAAAGGGTAGCCTCGAGTGAGACAAAATCGCAAAC1020.10814937336980723No Hit
TTTAGGGGGGCTTGCGTGCTGAGCTCAGTATGTGGCGTGTGCCCCGCAGGCTCTGT990.10496850944716585No Hit
TTTAGGGGGGGAGGCAAAGGGGCCCTGCTACCGAGTGCTGCATGATGGCGGATGCT970.10284793349873826No Hit
TTTAGGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC970.10284793349873826No Hit
TTTAGGGGGCCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC960.10178764552452445No Hit
TTTAGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCC950.10072735755031068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGATG204.0557695E-450.2676741
CATTGGC204.0557695E-450.2676749
ACATTGG204.0557695E-450.2676748
GTCTCAA402.5156623E-950.2676742
GCATGAT204.0557695E-450.2676740
GATTAAG402.5156623E-950.2676750
AGATTAA402.5156623E-950.2676749
AAAGATT402.5156623E-950.2676747
AATGAAG204.0557695E-450.2676750
CATCGAA204.0557695E-450.2676750
GTTGACA252.0082058E-550.26766645
CGATATG252.0082058E-550.26766650
TGACACA252.0082058E-550.26766647
CTTGGCA252.0082058E-550.26766640
TTGACAC252.0082058E-550.26766646
TCCCGAT252.0082058E-550.26766647
AGCGACT507.2759576E-1250.26766650
CCGATAT252.0082058E-550.26766649
GGAGCTA252.0082058E-550.26766650
CGTTCGC150.0082313850.26766242
Produced by FastQC (version 0.11.5)