FastQCFastQC Report
Tue 21 Mar 2023
SRR10540289_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540289_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155745404
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30086151.9317520278158578No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23259341.493420634101023No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14736760.9462083388348332No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8003210.5138649227812848No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG2797110.1795950267656052Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2049990.13162442982908182No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1958090.12572377416671635No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1820000.11685738090865269No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1653760.10618355068763377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG3463500.0137.687621
TCAATGA3471750.0136.002182
TAAGGTC1438900.0133.397252
AGGCTAA1186100.0132.509121
CAAGCTA1345600.0132.013432
CATCAAG1055200.0129.587941
ACAAGCT1394000.0128.48761
ACGCTTA1531350.0128.470872
CTAAGGT1505450.0128.208571
GGCTAAC1211200.0127.643492
ATCAAGT1087400.0126.534342
AGCTGAA1421950.0126.2670752
GATGTAC5963750.0126.245592
ATAGACA1846300.0126.001832
AGATGTA6020400.0125.239471
AACGCTT1590350.0125.1650851
AATCTGA1591400.0125.102762
AGCGTTA1504850.0124.660932
GAATCTG1610750.0124.645681
CCGAAGT1000050.0124.393141
Produced by FastQC (version 0.11.5)