FastQCFastQC Report
Tue 21 Mar 2023
SRR10540288_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540288_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137987586
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC51568333.7371716902127705No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA21777501.5782216814779266No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12832210.9299539452773672No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12726270.9222764430417675No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4015920.291034876137336No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2930860.2124002662094545No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG2781410.20156958177382708Illumina Single End PCR Primer 1 (100% over 22bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1893060.13719060205894174No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1496760.10847062720555167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC2214600.0138.16331
ATAGACA2306900.0137.000822
CTATGCA1695500.0136.584952
CAAGCTA1287100.0135.442022
ATGCCTA879600.0134.852221
CTCAATG1506550.0134.821561
GATGTAC3029150.0134.782332
GAGTCAA905850.0134.306212
ATGTTGC1686350.0134.20512
AGAGTCA919000.0133.873761
GAACTTA680050.0133.630142
ACAAGCT1314850.0133.46511
CGAACTT686150.0133.381961
AGTCACT778500.0133.348111
AGTTAGC616550.0133.006382
GGCTTCA985650.0132.855422
TAAGGTC687000.0132.571722
ACGCTTA975250.0132.559462
ATGAATC671250.0132.550382
GGCTAAC978500.0132.538592
Produced by FastQC (version 0.11.5)