FastQCFastQC Report
Tue 21 Mar 2023
SRR10540287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153812042
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6160460440.051873181684954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA45775092.9760407185804088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTAGCCGCATGCTGATAAG17377741.1298036079645832No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10082980.6555390507070961No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7670080.49866576766466697No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7519630.4888843488600197No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT7102780.46178308977914745No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7083080.4605023057947569No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG6808740.44266625105984875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC6359540.4134617756391271Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6146210.39959225039090246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA5995200.38977442351360236No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG5886370.382698904680038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA5469830.3556178000679557No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA5419980.3523768314577086No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5322330.3460281737888897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA5025290.3267162918232371No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5018120.3262501384644513No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4823640.31360613494748346No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4648400.3022130087837986No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA4324330.2811437871684975No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4270460.27764146060813627No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4037770.26251325627677446No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4010820.2607611177803621No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3863700.25119619697916756No Hit
AAGCAGTGGTATCAACGCAGAGTACACGTCTGAAAAAAAAAAAAAAAAAA3832570.2491722982261688No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA3707300.2410279424025851No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT3422200.22249233255742096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA3377030.21955563141148596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG3214340.20897843616171485No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA3154920.20511527959559891No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3125720.2032168586644211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA2965670.19281130147144138TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAG2871070.18666093776974887No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA2795700.1817608012771848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2753390.1790100413594405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAGCAGTGGTATCAACGCA2751550.17889041483501014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTAT2729900.1774828527404896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA2722720.1770160492375493No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2695520.17524765713727408No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2679900.17423213196792486No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2663150.17314314050911567No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2624940.1706589396947217No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2572440.16724568288352873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGG2445950.15902200947309444No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT2362010.15356469944011275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTACATGGGAAGCAGTGGT2330290.1515024421819977No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA2319630.15080938851328687No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2267200.1474006827111755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG2237130.14544569923855508Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG2167060.14089013914788284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC2162360.14058457139526176Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2116490.13760236015851085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG2064160.13420015579794461No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC1980320.12874934720650807No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1967930.1279438185990665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG1956120.12717599835258672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATTAACGCAG1668640.10848565419864852No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCA1651060.10734270077501473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGTAG1650210.10728743852188113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGT1582720.10289961562307325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAATGCAG1539060.10006108624446973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG786000.081.571562
GTGACTG795450.080.611551
GACTGGA909100.070.470463
GGAGTTC976700.065.198847
TGGAGTT982250.065.16766
AGTTCAG1025250.062.216599
CTGGAGT1103950.057.9639175
ACTGGAG1117400.057.336994
ACTATGC554600.056.9912151
CTATGCA731150.043.2000432
AAGCAGT369986700.040.6079751
AGCAGTG377825250.039.8285452
AGTGGTA379048750.039.71475
CAGTGGT381773850.039.422034
GCAGTGG382532100.039.3334273
TGGTATC385800550.039.008427
GTGGTAT387220650.038.879756
GTATCAA388300250.038.7716569
GGTATCA390161050.038.576268
GAGTTCA1964400.032.5963828
Produced by FastQC (version 0.11.5)