FastQCFastQC Report
Tue 21 Mar 2023
SRR10540286_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540286_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172803843
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC45768672.6485909806994283No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA28342651.6401631762321396No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA14560110.8425802197003223No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13533050.7831451989178274No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5148300.29792740199649376No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3368690.19494300251181335Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2449080.14172601473915136No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2143750.12405684750888325Illumina Single End PCR Primer 1 (100% over 21bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1846990.10688361832323369No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCA3288400.0131.009842
ACGCTTA1729150.0128.42982
GGCTAAC1310300.0128.37672
CGAACTT1173500.0127.195861
CAAGCTA1213850.0127.0568162
CTCAATG1864700.0126.574031
TGCCTAA1189250.0126.336652
CATCAAG1135250.0126.2837141
AACGTGA1899350.0126.122141
GATAGAC1769500.0125.695221
CAATGGA2948100.0125.593081
AGGCTAA1345850.0125.587881
ATGCCTA1216350.0125.4311451
ACGTGAT1918450.0125.227212
CCGAAGT1101750.0124.787361
AGTTAGC922000.0124.320192
TCAATGA1901050.0123.964332
GAGTCAA1247950.0123.9196852
ATAGACA1836750.0123.407392
ACAAGCT1280850.0121.6660461
Produced by FastQC (version 0.11.5)