FastQCFastQC Report
Tue 21 Mar 2023
SRR10540286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172803843
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6999880340.50766567731945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA42954222.485721338963509No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTAGCCGCATGCTGATAAG19453801.1257735743758892No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA12008530.694922623914099No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC9489920.5491729718071142Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9091360.5261086699327631No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8961410.5185885825467436No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT8643660.5002006813008204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG8409110.48662748779261816No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7702400.44573082787285007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG7365530.42623646975258533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG7016260.40602453499833335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA6920690.40049398669912684No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6905270.39960164543331367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA6199340.3587501233985867No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA6010530.34782386176446317No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5918670.342508007764619No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5892810.34101151326825524No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5445520.3151272509605009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA5395090.3122089130853415No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4603380.2663933810777576No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA4539450.2626938105768863No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4248090.24583307444152155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT4242490.24550900757455957No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4124820.23869955253252093No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA3855530.22311598706748667No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG3779590.21872140887514868No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3761670.21768439490087035No Hit
AAGCAGTGGTATCAACGCAGAGTACACGTCTGAAAAAAAAAAAAAAAAAA3730400.215874828663388No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3713960.21492346093252104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA3598240.20822685060308527TruSeq Adapter, Index 2 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAGCAGTGGTATCAACGCA3592910.20791840838863754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA3588650.20767188609341286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAG3559920.20600930732773112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT3493490.20216506411839466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG3338230.1931803102318737No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA3250580.18810808507308485No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA3242420.18763587335265455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA3240810.187542704128403No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3178520.1839380389242848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTAT3138930.18164700191303035No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3133590.18133798100774876No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA3027960.17522526972967842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC2989410.17299441656514547Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGG2984960.1727368991440775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTACATGGGAAGCAGTGGT2749050.1590850036824702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG2714040.15705900707312395No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2713270.1570144478789167No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2605150.150757642583215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG2544610.14725424827502245Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2512290.14538391949998475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC2422610.14019422010192215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA2353260.13618099916909834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG2286680.13232807559725393No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2276110.1317163993858632No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2228340.12895199327251072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGTAG2070980.11984571431087906No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2048290.11853266480884919No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGCAGTGGTATCAACGCAG2030120.11748118356372435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGT2007230.11615656024501725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCA2004800.1160159383723891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATTAACGCAG1975310.11430937910333396No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1903480.11015264284371268No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGTTATCAACGCAG1890380.1093945578513552No Hit
AAGCAGTGGTATCAACGCAGAGTACATTGGAAGCAGTGGTATCAACGCAG1889910.10936735938216373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1767090.10225987856068686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1015850.079.16191
TGACTGG1083500.073.913632
GACTGGA1205000.066.3474043
ACTATGC1300600.065.982321
CTATGCA1476600.057.87392
GGAGTTC1392500.056.9294327
TGGAGTT1390150.056.91696
CTGGAGT1500550.053.2903485
ACTGGAG1556500.051.4892964
AGTTCAG1603900.049.623449
AAGCAGT418878300.041.6540031
AGCAGTG424854600.040.9926072
AGTGGTA427374700.040.756125
CAGTGGT428479150.040.647594
GCAGTGG428364650.040.638593
GTGGTAT438678400.039.7022486
TGGTATC438792800.039.69847
TATGCAA671200.039.475483
GTATCAA445021000.039.220849
GGTATCA445470000.039.1079988
Produced by FastQC (version 0.11.5)