FastQCFastQC Report
Tue 21 Mar 2023
SRR10540284_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540284_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165875752
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC55572643.35025700441135No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA26978731.626442061284521No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23607891.4232273081119173No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12721110.7669059429493951No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3892620.23467082759630833Illumina Single End PCR Primer 1 (100% over 22bp)
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3857180.2325342886765029No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2976620.1794487719941128No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1942230.11708944656359417No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1795850.10826476916288524No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1666630.10047460101341393Illumina Single End PCR Primer 1 (100% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAA2149850.0133.299561
GGCTAAC2178950.0132.00932
AGTTAGC1202700.0130.058122
CTATGCA3056000.0129.579542
CAAGCTA1306100.0126.972332
CTCAATG1710750.0126.946941
GATAGAC2033900.0126.590041
CATCAAG1187500.0126.322531
AGATGTA6376150.0126.3166961
CGAACTT904550.0125.5326161
ATAGACA2091850.0125.486592
ACGCTTA1551750.0125.371432
GATGTAC6478600.0125.188872
ATCAAGT1228100.0123.305322
TCAATGA1764800.0123.002072
CAGATTC1497100.0122.979272
GCTAACT919950.0122.2096563
ACAAGCT1367550.0121.664161
CATTGGC1344600.0120.600432
GGCTTCA1584250.0120.395332
Produced by FastQC (version 0.11.5)