FastQCFastQC Report
Tue 21 Mar 2023
SRR10540282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141443446
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC55031713.8907218083473443No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23405401.6547532361449961No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12345430.8728173944517726No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11423110.807609707133408No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3893850.2752937735976823No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3490860.2468025277042529Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2641160.18672904787684544No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1842170.13024074653837264No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1499400.10600703266236881Illumina Single End PCR Primer 1 (100% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATC1573050.0132.613252
CGAACTT900250.0127.8052141
CAAGCTA1083450.0127.6200262
ACGCTTA1316250.0127.213342
CTCAATG1474800.0126.957681
ATGCCTA1084600.0126.764521
CAATGGA2871650.0126.14191
TGCCTAA1076300.0125.4508062
GATAGAC1523050.0124.75191
TAAGGTC825200.0124.22982
GGCTAAC1019550.0124.107512
CTATGCA1663050.0123.5056462
AGGCTAA1034650.0123.178771
CATCAAG857850.0123.13841
CTAACGA1172050.0122.678352
TCAATGA1509350.0122.439392
ATAGACA1598700.0121.8790052
TGGAACA1779400.0120.944671
ACAAGCT1154600.0120.7449951
GAGTCAA970900.0120.426152
Produced by FastQC (version 0.11.5)