FastQCFastQC Report
Tue 21 Mar 2023
SRR10540281_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540281_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178336573
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC66205883.7124118113450573No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA38594662.164147227388966No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA16597690.9306946814549364No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15544360.8716305207905952No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6928230.38849182102428315No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG4537500.2544346301865967Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3503670.1964639076023963No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2683620.15048063080140045Illumina Single End PCR Primer 1 (100% over 21bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2516550.14111238977324073No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1884860.10569116408892751No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCAAG1431500.0128.653411
ACGCTTA1799200.0128.509862
CAAGCTA1352600.0128.115332
AGGCTAA1459550.0127.95271
GGCTAAC1456800.0127.28972
ATCAAGT1483200.0126.8250662
CAATGGA3701950.0126.493781
GGCTTCA2078650.0125.154682
CTATGCA2171250.0124.811242
CTAACGA1719750.0124.247752
CTCAATG1569650.0124.112941
GATAGAC1885400.0123.748621
TGCCTAA1075000.0123.1680762
ATGCCTA1101250.0123.1351241
AGCGTTA1273300.0122.912882
CGAACTT827750.0122.763251
AACGTGA1601800.0122.476291
ACTTCGA1208950.0122.3857652
ACAAGCT1424000.0122.18841
ATAGACA1951300.0121.935512
Produced by FastQC (version 0.11.5)