FastQCFastQC Report
Tue 21 Mar 2023
SRR10540280_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540280_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150681222
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC55643793.6928151538351606No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA27415431.819432417398367No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA11917590.7909140795261137No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11469800.7611963752192028No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5049250.3350948401520131No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3666770.24334618151689796Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2594240.17216743835539108No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1857640.12328278038520286Illumina Single End PCR Primer 1 (100% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGC1299950.0131.478162
GGCTAAC1598650.0130.143882
ATAGCGA1725600.0129.941441
AGGCTAA1622200.0128.744131
TAGCGAC1741450.0128.638632
CAAGCTA1116200.0127.577132
CTATGCA1846750.0127.241842
ACGCTTA1251900.0126.4765552
CGAACTT901650.0125.804481
GATAGAC1482050.0124.511081
CAGATTC1302200.0123.885852
ATGCCTA881200.0123.885021
GAGTCAA1104700.0123.4431842
CGAGTAA1311850.0123.441082
CAATGGA2566450.0123.185971
ACGTGAT1428700.0122.814912
CCGAAGT854550.0122.665861
CTCAATG1229500.0122.493611
ACAAGCT1175700.0121.92341
ATGAATC802000.0121.813642
Produced by FastQC (version 0.11.5)