FastQCFastQC Report
Tue 21 Mar 2023
SRR10540279_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540279_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171705012
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC61892483.6045820258292753No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23702851.3804401935570758No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21191141.2341596644831776No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12170230.7087871144961104No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG4674340.27223084204437786Illumina Single End PCR Primer 1 (100% over 22bp)
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3770870.21961327488798058No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3534030.20581985108273948No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2203680.12834104108737374No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2166920.12620016007453527No Hit
CCTAATCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1844070.10739756391036506No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1788560.10416469380637532Illumina Single End PCR Primer 1 (100% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGT1729040.1006982836354247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC5073100.0139.464322
AGGCTAA5079400.0139.226361
GCTAACT2080000.0135.14993
ATGAATC1524900.0130.830182
CCTAATC1891350.0130.251
CAATGGA4151500.0129.2161
CTCAATG1778800.0128.282931
CTAATCC1930600.0127.427052
CAAGCTA1132000.0127.063812
CGAACTT964850.0126.970931
AGTTAGC1008000.0126.907982
ACGCTTA1470400.0126.796332
ATAGCGA1653600.0126.744351
GATAGAC1875700.0125.576541
GCTAACA1836850.0125.3507843
GAGTCAA1374800.0125.138512
TAGCGAC1670800.0124.904682
CTATGCA1740250.0124.264442
CATTGGC1508650.0124.111342
TCAATGA1820650.0123.74822
Produced by FastQC (version 0.11.5)