FastQCFastQC Report
Tue 21 Mar 2023
SRR10540278_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540278_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130859349
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC37022442.8291780665972897No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23884961.8252390969788486No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA14944791.1420498507905614No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11780020.9002046922914158No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4007250.3062257324847306No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1829210.13978443374343855No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG1815430.13873139472824367Illumina Single End PCR Primer 1 (100% over 22bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1790120.13679725703052367No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1760480.13453222971482154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCA1563800.0132.596972
AGTTAGC966600.0131.86622
GATAGAC1344550.0131.807591
AGATGTA3223450.0131.011141
CAATGGA3199700.0130.49291
CAAGCTA961750.0129.752152
GATGTAC3250400.0129.602142
CTCAATG1397400.0129.194351
ACGCTTA964800.0129.13292
ATAGCGA914550.0128.635481
ATGCCTA802650.0128.33761
ATAGACA1417600.0128.185532
GGCTAAC1036700.0127.925122
CCGAAGT881050.0126.6163561
CGAACTT784150.0126.5231551
AGGCTAA1065850.0125.997451
TAAGGTC844900.0125.724012
TAGCGAC928750.0125.302592
TCAATGA1431900.0125.280332
AGAGTCA854850.0125.094371
Produced by FastQC (version 0.11.5)