FastQCFastQC Report
Tue 21 Mar 2023
SRR10540277_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540277_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161094600
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC52065773.2319997070044555No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA47531752.9505489321181466No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12441220.7722928018692123No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA11311560.702168787780596No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG8819380.5474658989190202No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3238160.20100984142236925Illumina Single End PCR Primer 1 (100% over 22bp)
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA3052670.1894954889859747Illumina Single End PCR Primer 1 (100% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2513220.15600895374519072No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2167020.134518475479625No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2024960.12570005450213725No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2014860.12507309369774033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGC1025000.0127.347382
CGAACTT1068650.0127.177011
ACGCTTA1195800.0126.271072
CTCAATG1531650.0126.077241
GGCTAAC1316100.0125.9503252
GATAGAC1580350.0125.601731
CATCAAG1066100.0124.812111
CGACTGG3677500.0124.703371
TCACGCA1813750.0124.6012952
CAAGCTA941850.0123.078212
ATAGCGA1135400.0122.859981
ATAGACA1650400.0122.859122
GGCTTCA1817700.0122.7499542
CTATGCA1492000.0122.732232
AATCCGT1165450.0122.6838461
CAATGGA2660500.0122.642691
ATCAAGT1110000.0121.079052
CGAGTAA1160600.0120.968282
AACAGGC2782300.0120.840312
TCAATGA1597550.0120.577362
Produced by FastQC (version 0.11.5)