FastQCFastQC Report
Tue 21 Mar 2023
SRR10540277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161094600
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6049158937.55035178087906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA34199582.1229501175085943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTAGCCGCATGCTGATAAG12314380.7644191673712216No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA9785750.6074536328343718No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7740450.4804909661776372No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7652800.475050063751361No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT7582860.47070851536923025No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7064190.43851190542699753No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC7041250.43708789742176335Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6231990.3868528181577781No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG5780530.35882829095450747No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5676340.3523606626168723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG5535090.3435925226543906No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA5503250.34161604423736114No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5029630.3122159277840474No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4981990.3092586591977633No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4678620.29042686719480354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA4660980.2893318584235598No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4465110.27717316409116133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG4450120.27624265493691286No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA4430180.27500487291318265No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4231560.26267547143107217No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4173350.25906206663662223No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4064680.25231634083327437No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA3953200.24539618336058439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA3950130.2452056121061786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA3656270.22696415646458662No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3260960.2024251588817999No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT2823750.17528520509067344No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2809010.17437021476821693No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA2798270.1737035257544325No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2762560.1714868158212628No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2745750.1704433295715685No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2731660.16956868821177123No Hit
AAGCAGTGGTATCAACGCAGAGTACACGTCTGAAAAAAAAAAAAAAAAAA2703780.16783802809032705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA2700720.16764807758919295TruSeq Adapter, Index 2 (95% over 22bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2692460.16713533538678516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC2679070.16630414675600547Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2650080.1645045830214048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG2587070.16059321665654838Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2570500.15956462848537445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAG2493050.15475689439621193No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2413430.14981445684709482No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA2328230.14452563897238022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTAT2298050.1426522055984496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGGAAGAGCACACGTCT2258810.14021636976037682Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2184480.1356023106919785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA2179310.1352813812505199No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAGCAGTGGTATCAACGCA2145270.13316833711372075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG2118020.13147678444839242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT2100690.1304010190285708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG2029610.1259887047734685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGG2005810.12451131198686982No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1997100.12397063588723645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTACATGGGAAGCAGTGGT1948290.12094073916816578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA1942120.12055773439953917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGCAGTGGTATCAACGCAG1818210.1128659806101508No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA1798600.11164868344438608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG1707540.10599610415246695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG943550.068.602951
TGACTGG972800.066.436622
GACTGGA1108950.058.236743
TGGAGTT1265950.050.5820776
GGAGTTC1319100.048.359847
AGTTCAG1406150.045.550359
ACTGGAG1418400.045.5465554
CTGGAGT1430800.045.111575
ACTATGC591500.044.808361
AAGCAGT349111750.043.658181
AGCAGTG356915800.042.719082
AGTGGTA358283550.042.559115
CAGTGGT360663250.042.280734
GCAGTGG361540400.042.169213
TGGTATC369147100.041.310157
GTGGTAT369618800.041.2578546
GTATCAA371201800.041.0916069
GGTATCA373658400.040.8144268
CTATGCA756350.035.0136342
AGCGTTA431150.027.8725172
Produced by FastQC (version 0.11.5)