FastQCFastQC Report
Tue 21 Mar 2023
SRR10540276_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540276_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145666891
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC44515593.0559854538256057No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA22139011.5198381628121658No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13940740.9570287320816094No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11922850.8185010278004766No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3856410.26474169754882737No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3242160.22257357026999358Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2103780.14442403387328423No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1777560.12202910268744598No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1663370.11418998432526442Illumina Single End PCR Primer 1 (100% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC1844300.0132.343052
AGGCTAA1858550.0132.194441
AACGTGA2113300.0132.027831
ACGTGAT2134200.0130.98232
ACGCTTA1438250.0130.661652
CGAACTT1141800.0130.43411
CTCAATG1758800.0130.181351
CTATGCA1894400.0129.751482
GAGTCAA1552600.0129.358292
AGAGTCA1583150.0128.394211
CAAGCTA1067900.0128.359072
GATAGAC1389450.0125.798691
TCAATGA1811950.0124.568552
ATAGCGA1094050.0124.5448761
TAAGGTC860500.0124.260822
GCTAACT756500.0123.9505543
ATGCCTA820350.0123.9301151
AGCTGAA1328750.0123.855582
ATAGACA1450500.0123.427492
CATCAAG814000.0122.8348851
Produced by FastQC (version 0.11.5)