FastQCFastQC Report
Tue 21 Mar 2023
SRR10540275_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540275_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174281883
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC48783342.7991056305032003No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA18969121.088416057565777No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13208280.7578687912156653No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13137140.7537868982055926No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3005530.17245223360365Illumina Single End PCR Primer 1 (100% over 22bp)
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2613960.14998460855509577No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1923490.11036660649345864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC2836550.0133.004212
AGTTAGC1759350.0132.602112
AGGCTAA2860900.0132.160611
CGAACTT1445500.0129.145191
ACGCTTA1572350.0128.974182
ACGTGAT2098550.0128.746642
AACGTGA2110900.0127.782851
CTATGCA2102450.0126.069392
CGAGTAA1858200.0125.90852
GCTAACT1169900.0125.538073
CCGAAGT1253750.0125.3532261
CAATGGA3462750.0123.963271
GATAGAC1735050.0123.920721
TCACGCA1899250.0123.035492
ACTTCGA1104350.0122.818192
CGCATAC2011600.0122.811431
CTCAATG1676950.0122.398311
CAAGCTA1138300.0122.349532
AATCCGT1313400.0119.813681
GCGAGTA1973850.0119.773981
Produced by FastQC (version 0.11.5)