FastQCFastQC Report
Tue 21 Mar 2023
SRR10540272_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540272_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153144556
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC44446022.902226573434318No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA33534812.189748749540924No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13087450.8545814713779314No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11087590.7239950468758419No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6062890.39589327615406716No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3067120.20027613648897843Illumina Single End PCR Primer 1 (100% over 22bp)
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2360550.15413868188693564Illumina Single End PCR Primer 1 (100% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2131380.13917438893485706No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2064440.13480335533442012No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1994350.13022663371723117No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1756290.11468184347343043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAA1519500.0130.188541
GGCTAAC1521050.0129.482392
CGAACTT986200.0128.769971
CTCAATG1845600.0128.613951
CAATGGA3321800.0127.6543051
AGTTAGC975000.0127.4532552
ATGCCTA1134950.0126.533241
CAAGCTA1028900.0126.48012
GAGTCAA1321850.0125.827662
TGCCTAA1121800.0125.447822
CTAACGA1721050.0125.2087862
TCAATGA1862500.0125.163762
ACGCTTA1265400.0125.101852
GATAGAC1613200.0124.55551
CTATGCA1726750.0123.6028442
AGAGTCA1365450.0123.3079151
ATAGACA1677200.0122.4246442
ATAGCGA1186200.0122.201791
CATTGGC1337050.0122.081482
AGATGTA3442050.0120.989151
Produced by FastQC (version 0.11.5)