FastQCFastQC Report
Tue 21 Mar 2023
SRR10540271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156042129
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC122652617.860224080895486No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA42590572.7294276406597864No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21482331.3767006472976282No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA15581540.9985470013678164No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATAC8548450.5478296185000142No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6800090.4357855178969008No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC6412790.4109652977113636No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5985680.3835938434292959Illumina Single End PCR Primer 1 (100% over 50bp)
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG5820650.3730178534029102Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC3750380.2403440675947199No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1748440.11204922742370427Illumina Single End PCR Primer 1 (100% over 21bp)
CATGTACTCTGCGTTGATACCCCATGTACTCTGCGTTGATACCACTGCTT1729770.11085275566831058No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCTTGTACTCTGCGTTGATA1671730.10713324732963621No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGC2001900.0131.642122
CCGTCTA2983550.0122.0787352
AGGCTAA1289250.0121.9898151
GGCTAAC1289800.0121.814992
TAGCGAC1188050.0118.6794052
CCTAATC1556450.0118.6666261
ATAGCGA1196100.0118.103381
CTAATCC1582650.0117.1115652
GATGTAC3483900.0116.9432142
AAGGAGC1596150.0116.599182
ACGCTTA1037350.0115.022952
AAGGACA1979400.0114.843041
CTAACGA1174850.0114.497522
GAGTTAG2275950.0114.332611
AGGACAC1998800.0113.5699162
TCACGCA1223950.0113.0337142
TCTTCAC6082850.0112.925221
CTATGCA1245750.0112.899282
CGACACA2543900.0112.645421
AGATGTA3642250.0111.732491
Produced by FastQC (version 0.11.5)