FastQCFastQC Report
Tue 21 Mar 2023
SRR10540269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157510336
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC72165614.581642819935321No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA28386471.8021972856435273No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA26805901.7018502201658692No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17182461.0908782519516687No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3890350.24699014038037478No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3713720.23577627312026053No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3707160.23535979251545752Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC2199620.13964924816108576No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1871420.11881252034152223No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1583470.10053118037917208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGTA5497150.0136.772431
ATAGCGA1238400.0136.139911
AGTTAGC1349450.0136.109152
GATAGAC1778650.0136.010851
GATGTAC5570850.0135.143772
ATAGACA1843750.0134.375872
GGCTAAC1101750.0132.447532
AGGCTAA1116050.0131.353231
CAAGCTA1037350.0131.333222
CGAACTT1020700.0130.892871
TAGCGAC1288200.0130.685032
CTCAATG1559400.0130.588361
CAATGGA2679700.0129.83821
GAACTTA1033150.0128.981522
AACATCG1397300.0128.855872
GAGTCAA794150.0128.508062
AGAGTCA799800.0128.289811
TCAATGA1585500.0127.935632
ATGCCTA841950.0127.9345551
ACGCTTA948350.0127.8339542
Produced by FastQC (version 0.11.5)