FastQCFastQC Report
Tue 21 Mar 2023
SRR10540268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148686560
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC71495834.808493114643314No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA22323311.5013670368054786No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18366171.2352273130806173No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA15427841.037608241121457No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3394510.2282997198939837No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3278210.22047789658998096Illumina Single End PCR Primer 1 (100% over 22bp)
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2956430.1988363978559999No Hit
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC1876670.1262165188299467No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1652720.11115463294059665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA975350.0134.440441
GGCTAAC1300500.0133.775182
GATAGAC1135900.0133.59711
GATGTAC3131800.0132.374742
CAATGGA2782150.0131.986921
ATAGACA1178500.0131.256442
AGGCTAA1326100.0131.162861
AGATGTA3152500.0131.017821
CAAGCTA918750.0130.772352
AGTTAGC793300.0130.731642
CTCAATG1268950.0129.515931
ACGCTTA926300.0128.78152
AACATCG1202900.0128.665822
ATGCCTA850350.0128.582931
TAGCGAC1019350.0128.426122
CGAACTT906550.0127.944321
CTATGCA1043850.0127.2811052
GAACTTA914250.0126.887692
GCCACAT1511300.0126.774511
GAGTCAA634400.0126.534162
Produced by FastQC (version 0.11.5)