FastQCFastQC Report
Tue 21 Mar 2023
SRR10540264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199013503
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG185340909.312981139777234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG78996363.969396991117733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA25817721.297284838004183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17815310.8951809666904863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG9139690.4592497424659672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTAGCCGCATGCTGATAAG8236240.4138533253193378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA6031620.30307591741651824No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4866170.24451456442128955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGTGAGATGGCTCAGT4130000.20752360707906337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGTTGGGGATTTAGCTCA2771690.1392714543595567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTGGGGATTTAGCTCAG2621540.13172674016998737No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2325640.11685840231655034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2231300.11211802045411963Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG2131250.10709072338674426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT162502200.074.447741
AGCAGTG164764650.073.3942262
AGTGGTA165503400.073.071675
GTATCAA166611700.072.612039
GCAGTGG166904150.072.441423
TGGTATC167320200.072.27357
GTGGTAT167457300.072.2199256
GGTATCA167481600.072.2059568
CAGTGGT168784550.071.66224
GCGTAGA12729000.026.69011355-59
AGCGTAG12804500.026.64115555-59
AAATCGC12708100.026.506474105-109
AATCGCA12723400.026.464413105-109
GAAATCG12748300.026.333988105-109
AGCCCGA12888400.026.31585795-99
CAGCCCG12990450.026.22319495-99
GCAGCGA12808950.026.15144740-44
GGCATTC12996550.026.10599930-34
CGTGAGG13011700.026.07141780-84
TCGTGAG12911900.026.0680480-84
Produced by FastQC (version 0.11.5)