FastQCFastQC Report
Tue 21 Mar 2023
SRR10540263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196899618
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC63311663.215428279804992No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA36990671.8786562602676051No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20153191.0235261096341994No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA15011480.7623925405482503No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6872190.34901997626018755No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3808600.19342851137476558Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3129150.1589210802836601No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2372670.12050150346152526Illumina Single End PCR Primer 1 (100% over 21bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2127640.10805709130426043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC8677500.0141.555471
ATAGACA8980900.0139.938062
ACGCTTA3164550.0137.452872
TAGACAT3524000.0134.687183
CAAGCTA1956700.0132.637342
AACGCTT3316300.0132.127791
AACGTGA1945200.0132.043381
CGAACTT1653400.0131.975921
AGTTAGC1220850.0131.281432
ACGTGAT1965850.0131.038472
GTCGTAG2160850.0130.875171
TAGACAA2955650.0130.384613
AACATCG2201700.0130.343442
GGCTAAC1692150.0129.831852
ACTTCGA1380650.0129.504262
AGTCACT1765800.0129.146971
AAGGACA3950650.0129.054241
GTACAAG1769000.0128.896532
GACTAGT1940650.0128.678631
GTCACTA1762100.0128.367512
Produced by FastQC (version 0.11.5)