FastQCFastQC Report
Tue 21 Mar 2023
SRR10540262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148136917
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6105547841.215572212833344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA43888352.962688227135171No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG21146421.4274915684926803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGAAGCAGTGGTATCAACGCAG16381461.105832383429446No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA13184680.890033373652565No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA9889420.6675864598964213No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT9767320.6593440850399229No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT8973310.6057443466303541No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8839160.5966885351070186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC8735660.5897017554375051Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG8511610.574577233843742No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT7428230.5014435395600949No Hit
AAGCAGTGGTATCAACGCAGAGTACATCTAGATCGGAAGAGCACACGTCT6417570.43321881742685386Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA6330770.42735937322092366No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6052370.40856594848669625No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5774520.38980965156713776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA5304860.35810519804459007No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5292920.3572991869406868No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA5052030.3410378791668791No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4982440.33634019803449805No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4834010.32632041343212237No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4409420.29765841555889816No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA4327040.2920973439726709No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG4163290.2810433809689721Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3973740.26824778593171344No Hit
AAGCAGTGGTATCAACGCAGAGTACACGTCTGAAAAAAAAAAAAAAAAAA3925780.2650102404925843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA3924400.2649170834303241No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3837370.25904211304735064No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3457890.23342527102815294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAACAAAACAAAACAAA3394970.2291778490300294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA3215220.2170438041450532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG3182330.21482356082785226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA3159980.21331482144994282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG2904960.19609966636473203No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2823540.19060339969138146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGATCGGAAGAGCACACGTCTG2818030.19023144649351653Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA2706680.18271475165100134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAGCAGTGGTATCAACGCA2695630.18196882010174412No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2692740.1817737303119384No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2505130.1691090952027846No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2467310.16655605165591506No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2460440.16609229149814156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC2448160.1652633286542611Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2368310.1598730450155109No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2243220.1514288298574487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA2195390.14820006008360495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAA2094020.14135706631453657No Hit
AAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAG1889740.12756712089532685No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1870790.1262878989171889No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG1649730.11136521762498947No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1627260.10984837763297045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGCAGTGGTATCAACGCAG1595030.10767268769337221No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT1571760.10610184360728932No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA1528760.10319912355135621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC297950.054.4175221
GATGTAC256750.040.5630382
AAGCAGT380943900.040.3022351
AGCAGTG388497400.039.4653432
AGTGGTA391519850.039.16355
GCAGTGG392274900.039.041283
CAGTGGT394079200.038.890974
GTGGTAT398581150.038.4727826
TGGTATC398559850.038.470167
GTATCAA402685950.038.141669
GGTATCA402866250.038.0698
CTATGCA449150.035.8873442
AGATGTA390650.027.2148021
TGACTGG213800.024.6927192
ATTGAGC841250.022.567894135-139
AGCCCGA813650.022.16978695-99
GTGACTG238850.022.0142671
GATAAGT820850.021.938547115-119
AATCGCA836700.021.420658105-109
GGCATTC795400.021.40915930-34
Produced by FastQC (version 0.11.5)