FastQCFastQC Report
Tue 21 Mar 2023
SRR10540260_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540260_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170236334
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC67516103.9660217307076175No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA27531261.6172375986433074No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA11724200.6887013908558439No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9137040.5367267836019072No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC5666300.33284903797329185No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC5379090.3159777865047305No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG4786250.28115325838724886Illumina Single End PCR Primer 1 (100% over 22bp)
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4007050.23538159603460446No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3876180.2276940479698065No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2027200.11908151170595581Illumina Single End PCR Primer 1 (100% over 21bp)
CCTAATCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1825810.107251487217764No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1763710.10360361731003911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGA1908950.0130.450942
ACGTGAT2004900.0129.487782
AACGTGA2001300.0129.143861
AACATCG2523100.0127.444282
GGCTAAC1235450.0126.782182
CGAACTT1267300.0126.718211
ACGCTTA1594500.0126.5729142
TCACGCA1896700.0125.408582
AGGTACA2029500.0125.310262
AATCCGT1405600.0125.235521
CAAGCTA1197000.0124.85672
ATAGCGA1125200.0124.340611
AGTTAGC842300.0123.214452
CTAACGA1418150.0122.92522
AGCAGGA3092900.0122.910711
CCGAAGT1440200.0122.808241
TAGCGAC1139450.0122.7510452
AGATGTA3582550.0122.165191
ACGTATC2251750.0122.0347751
GATAGAC1200050.0121.800561
Produced by FastQC (version 0.11.5)