FastQCFastQC Report
Tue 21 Mar 2023
SRR10540258_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540258_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167460624
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC108231616.463108008005512No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA99791565.959105944810047No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA37278602.226111375292618No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14466300.8638627788703331No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG8266440.49363484994538176No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG8071740.48200823615705624Illumina Single End PCR Primer 1 (100% over 22bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC7714450.4606724742647561No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG7546510.45064384807260727No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7311840.4366304045302017No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC6001650.35839171362457123No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGCGTTGATA5753010.34354404412108247No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA5621760.33570638074297393Illumina Single End PCR Primer 1 (100% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGCGTTGATAC5496960.3282538825365896No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC4233430.2528015182840833No Hit
CATGTACTCTGCGTTGATACCACTGCTTCTCATGTACTCTGCGTTGATAC3979160.23761765034388027No Hit
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3914190.2337379323273034No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCTCATGTACTCTGCGTTGATA3825950.22846863391599448No Hit
TATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3406210.20340363714397722No Hit
CTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC3236890.19329260351973848No Hit
CATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATAC3058960.1826674191778958No Hit
AGATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATA2932220.17509907284234172No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2855630.17052546036135635Illumina Single End PCR Primer 1 (100% over 21bp)
CCTAATCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2676500.15982861738291385No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2350520.1403625487505648No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGCGTTGATA2012080.1201524246081873No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1994630.11911038860096448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGTA20206400.0139.46651
GATGTAC20753800.0135.768072
CTCAATG1483750.0130.131851
CAATGGA3314700.0127.143771
TCAATGA1506800.0126.820092
CGAACTT728650.0126.3787841
CCTAATC1382900.0125.179141
CAAGCTA887250.0124.693172
GATAGAC1486750.0123.448351
ATAGACA1530250.0122.848252
CTAATCC1400200.0122.690822
ATCCTCA1899550.0122.3570563
GAACTTA762500.0120.1873862
AGGCTAA657850.0119.666421
ACAAGCT933700.0118.524321
ACGCTTA888250.0118.520552
GGCTAAC661700.0117.5774842
ATCATTC2448700.0116.34241
AATCCTA680650.0116.333654
AATGGAA3631700.0115.8312152
Produced by FastQC (version 0.11.5)