FastQCFastQC Report
Tue 21 Mar 2023
SRR10540257_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540257_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155164983
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC72568544.67686320695179No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA21112711.3606620251426187No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20529371.323067202604598No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC4625860.2981252542012008No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC3949490.25453487788543117No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3812970.24573650099906885Illumina Single End PCR Primer 1 (100% over 22bp)
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3617470.233137008754095No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3408320.21965780771554624No Hit
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC3023530.1948590423910271No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2062600.13292947674927402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2707950.0139.534031
TAGCGAC2743450.0138.020582
AGTTAGC1434450.0134.582492
AACATCG1548750.0131.715582
GATAGAC1185200.0131.40651
GGCTAAC1150100.0130.26162
ACGCTTA1156500.0130.076082
AGGCTAA1152550.0129.48591
CGAACTT1111150.0129.383941
CAAGCTA965050.0128.734632
ATAGACA1238950.0128.630782
CTCAATG1625250.0128.134321
TATCAGC1367400.0127.0131
AACGTGA966500.0126.786071
TCAATGA1641900.0126.2248152
GCTCATG3120650.0125.807831
CAATGGA2320650.0125.73491
ATCAGCA1374350.0125.481232
CTATGCA1295950.0125.3232
CTAACGA1267350.0124.9871142
Produced by FastQC (version 0.11.5)