FastQCFastQC Report
Tue 21 Mar 2023
SRR10540256_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540256_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133416277
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC62969604.719783928613149No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19773781.4821115117760333No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19638861.4719988026648354No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12439280.932365996092066No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA4757460.3565876748307105No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG3038650.22775706745287158Illumina Single End PCR Primer 1 (100% over 22bp)
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2993890.22440215446875347No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2760880.20693726898105544No Hit
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC1443900.10822517555335472No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1342950.10065863252952262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1234050.0136.594351
ACGCTTA1629850.0136.307742
GGCTAAC1414900.0135.752992
CTATGCA1875100.0135.118932
GAGTCAA1214950.0135.063372
AGAGTCA1223900.0134.661561
AGGCTAA1431850.0134.035371
AGTTAGC928900.0133.547762
CAATGGA2720450.0133.141191
CGAACTT1101650.0132.33431
TAGCGAC1272500.0132.129522
ACGTATC1100100.0131.447171
AACGCTT1703200.0130.896181
GAACTTA1120950.0130.767032
CTCAATG1254400.0130.176011
AGCTGAA1213800.0130.077682
AGATGTA2780950.0129.377181
AACCGAG1648350.0129.275221
GATGTAC2800450.0129.060912
TAAGGTC979300.0128.851142
Produced by FastQC (version 0.11.5)