FastQCFastQC Report
Tue 21 Mar 2023
SRR10540256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133416277
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5628817642.189886620805645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA31146942.3345682176395917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTAGCCGCATGCTGATAAG10877200.815282830894764No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10642550.7976950218750296No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7529690.5643756645975063No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7418430.5560363522960545No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT7127510.5342309169667506No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7032840.5271350811265705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC6657710.499017822240685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG6317670.4735306772201415No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6193230.4642034794600063No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA5574210.4178058423860831No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5553760.41627304590428643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG5377930.4030939942957635No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5133230.3847529038754394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG5129100.3844433464441524No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4643830.3480707230347913No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4367010.3273221302675085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA4326040.3242512905677918No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA4263190.3195404710626125No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4221150.3163894312535794No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4044480.30314741881157425No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3823250.28656548406008964No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA3582830.26854519407703153No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3526540.26432606869999825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA3271820.2452339454802805No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3082970.23107900095278477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT2845770.21330006083140812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGAAGAGCACACGTCTG2699560.20234112813686142Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA2680370.20090277290528802No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA2586080.19383541934692122No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2580690.19343142066541102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAAAAA2549370.19108388101700666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC2511700.1882603874488268Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTAT2472810.18534545076535153No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2448330.18351059218958718No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2387400.17894368316093845No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2383870.17867909775356722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2334760.1749981375960596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGG2331520.1747552886669143No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2295090.17202473728149376No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAG2231800.16728093829210958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTACATGGGAAGCAGTGGT2094710.15700558036108292No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2048810.1535652205315248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG1945200.14579930153499937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC1864120.13972208203651193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT1855060.13904300447538345No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1852890.13888035565555468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA1798750.13482238003088634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTG1755100.13155066529101242No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1677160.12570879938435098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAG1616560.12116662496885594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1556100.11663494402560791No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1528020.11453025330634883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCA1510100.11318708885873048No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG1503160.11266691244877114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGCAGTGGTATCAACGCAG1487420.11148714635471353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAAAAAA1457780.10926552837327339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCA1428010.10703416645331813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAGCAGTGGTATCAACGCA1427640.10700643370523673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1345100.10081978228188755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAGT1341060.10051697065418785No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC806100.065.8568341
GTGACTG699550.061.342331
CTATGCA873550.060.6810762
TGACTGG734900.058.0977672
GACTGGA804500.053.0702743
TGGAGTT981350.044.9575046
GGAGTTC1020800.040.89267
CTGGAGT1058950.040.2896585
AGTTCAG1053300.039.692239
AAGCAGT332932350.039.3383641
ACTGGAG1088550.039.2880974
AGCAGTG339785000.038.534872
AGTGGTA341302850.038.3691145
CAGTGGT343233950.038.148494
GCAGTGG344149600.038.035443
TGGTATC350123600.037.406537
GTGGTAT350731950.037.340146
GTATCAA351995900.037.2268039
GGTATCA354310050.036.9675758
TATGCAA459200.035.870173
Produced by FastQC (version 0.11.5)