FastQCFastQC Report
Tue 21 Mar 2023
SRR10540255_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540255_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165449444
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC64949273.9256263683787296No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA38525782.3285530049892462No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19580831.1834932488500838No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13055430.7890887804978057No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6797150.41082942533188566No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG4386430.26512207559911777Illumina Single End PCR Primer 1 (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2980240.18012994954519157No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA2629260.15891621853984592Illumina Single End PCR Primer 1 (100% over 21bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2545020.15382463297972765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG1985150.0134.701082
GGCTAAC1583600.0134.631762
ACGCTTA1282150.0133.26872
AGGCTAA1600950.0133.26321
CAAGCTA1112000.0132.78392
GATAGAC1402250.0132.379851
CTCAATG1781900.0131.798081
CGAACTT1117400.0131.279621
ATAGACA1455700.0131.131532
GATGTAC2919100.0130.82512
AGTCACT1245600.0130.58551
AGGTACA1451400.0130.488112
GAGTCAA962650.0130.062032
AAGGTAC1468300.0129.303861
ATGCCTA903850.0129.239791
AGTTAGC838000.0129.232912
GTCACTA1251450.0129.207642
ACAAGCT1152200.0128.70211
AGAGTCA978800.0128.650511
AGCTGAA1299200.0128.310752
Produced by FastQC (version 0.11.5)