FastQCFastQC Report
Tue 21 Mar 2023
SRR10540254_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540254_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153070759
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC41215822.6925991789196004No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23137241.511538856353355No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14280600.9329410851095341No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4480900.29273389831430835No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2869080.18743488428119703No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG2706630.17682214537134425Illumina Single End PCR Primer 1 (100% over 22bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2271760.14841240840780048No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1999830.13064742169338822No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1555820.10164057525840059Illumina Single End PCR Primer 1 (100% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2008900.0138.86611
AACATCG2481550.0136.995572
TAGCGAC2045950.0136.125722
GATAGAC1471000.0134.02451
CAAGCTA1233800.0133.940322
ACGCTTA1344200.0133.560242
CGAACTT1153800.0133.406891
GGCTAAC1019700.0131.97152
AACGTGA1343750.0131.560031
AGTTAGC1031950.0131.535632
CTATGCA1489150.0131.312272
ACGTGAT1360850.0130.851672
CAATGGA2841950.0130.810941
ATAGACA1548700.0130.421042
AAGGTAC1517950.0130.154421
AGGTACA1509800.0130.05062
ACAAGCT1279900.0129.92771
ATGCCTA994050.0129.43711
GAACTTA1190250.0129.394582
TCACGCA1706800.0129.147892
Produced by FastQC (version 0.11.5)