FastQCFastQC Report
Tue 21 Mar 2023
SRR10540252_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10540252_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163776853
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC153019009.343139594946303No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA21542541.315359258979045No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13278440.8107641438195177No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC9792190.5978982878612278No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG7920620.48362267652071694Illumina Single End PCR Primer 1 (100% over 22bp)
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3850980.23513579174707916No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3839860.23445681912083144No Hit
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC3413730.20843787980222092No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2498980.1525844436637209No Hit
CCTAATCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2140460.13069368233617237No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCCGTTGATAC2020590.12337457723650361No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1764450.10773500453082953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA3447650.0139.570652
TATCAGC3166700.0137.107161
CGAGTAA2087150.0135.192262
ATCAGCA3213100.0134.579252
AACGCTT3593050.0134.486391
GTACAAG2212450.0134.22952
GGCTAAC1809500.0133.832552
GCGAGTA2146050.0133.093411
AGTTAGC1395600.0132.998522
TCACGCA2029550.0132.790312
GTGCGAA1659450.0132.718922
AACCGAG2549400.0131.922261
GTCACTA1660450.0131.805662
CGCATAC1760450.0131.653471
AGTCACT1673000.0131.423741
AGTACAA2279550.0131.416431
ACCGAGA2523700.0131.373022
CAAGCTA1434150.0131.353122
AAGGACA3806600.0130.978321
CAAGACT2254900.0130.975981
Produced by FastQC (version 0.11.5)