FastQCFastQC Report
Tue 21 Mar 2023
SRR9602599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69313337
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA50835947.334221983858605No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24175453.487849676029881No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC6267120.9041723095801893No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC4321520.6234759697112837No Hit
CTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC3524830.5085356083779374No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3263230.4707939541274719No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2345820.3384370312455163No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGCGTTGATA1369890.19763728876594128No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1231290.1776411370873689Illumina Single End PCR Primer 1 (100% over 21bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1070520.1544464667744968No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG828820.1195758328588335Illumina Single End PCR Primer 1 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGTA8758800.0141.212941
GATGTAC8824850.0140.273062
AACGTGA1798650.0139.590731
ACGTGAT1815650.0138.179022
AAGGACA1936950.0136.761671
AGGACAC1947750.0136.07292
AGGTACA1564100.0135.57072
CGTGAGA1398950.0135.143052
AGCAGGA1078450.0132.844761
CCGTGAG1431800.0132.719061
AGAGTCA685050.0132.687531
AGTTAGC628350.0132.411772
CAATGGA1976850.0132.342221
GTCACTA988950.0132.272832
AGTCACT989300.0132.072161
AACATCG1117300.0131.4132
GAGTCAA691250.0131.239782
ACAGCAG1568600.0129.788421
CAGCAGA1564150.0129.608492
TGGCATA865450.0129.579382
Produced by FastQC (version 0.11.5)