FastQCFastQC Report
Tue 21 Mar 2023
SRR9602598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58845296
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA26243244.459700568079392No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20232763.4382969201140563No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA11336671.9265210255718657No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4483340.7618858778448493No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2648420.4500648615991328No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2443480.4152379486713772No Hit
CTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2045180.3475519946403193No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1892890.3216722709662298No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG975600.16579065215340236No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC971870.16515678670390238No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC928320.15775602522247487No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGCGTTGATA823680.13997380521290947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAGG5432650.0140.556091
AACAGGC5457450.0140.21692
AGATGTA4383100.0139.08971
ACAGGCT2519150.0137.146843
GATGTAC4565050.0134.37892
CAATGGA2722100.0134.314471
CTACCGT2017550.0133.813672
ACCGTGA1335600.0132.705384
CTCAATG1328250.0131.180731
TCAATGA1337200.0130.740222
GCTACCG2705500.0130.65531
AAGGACA1261150.0130.604661
AGCAGGA1323600.0130.315281
ACAGGCA1630450.0129.966753
ACAGGCG1609200.0129.857993
CCGTGAC1515850.0129.437475
AGGACAC1282450.0129.227022
CAGGCTA740450.0128.424044
AATGGAA2872650.0128.270262
CGTGACT1108400.0127.53856
Produced by FastQC (version 0.11.5)