FastQCFastQC Report
Tue 21 Mar 2023
SRR9602597_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602597_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75193311
Sequences flagged as poor quality0
Sequence length150
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1853700.24652458780542327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT4460500.0141.912112
AACGTGA4480700.0141.726841
GATCGCA2599150.0141.507972
AGATCGC2622100.0141.471221
CGTGAGA2839800.0140.945272
ACGTATC2358250.0140.829941
CCGTGAG2864000.0140.611631
CGCATAC2733800.0140.519641
GACTAGT2150550.0140.245651
AACATCG3733300.0140.151112
ACGCTTA3052400.0140.05132
ACCGAGA1903650.0139.514742
AGAGTCA2181050.0139.459961
GTCACTA2506400.0139.442612
AGCAGGA3146550.0139.035491
GAGTCAA2181550.0138.814582
AACCGAG1944650.0138.496231
AGTCACT2538050.0138.443731
AGTGGTC2854900.0138.42371
GCATACA2773350.0138.312592
Produced by FastQC (version 0.11.5)