FastQCFastQC Report
Tue 21 Mar 2023
SRR9602597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75193311
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG19634962.6112641854539427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9557371.271039920026929No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4908490.6527827987252749No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2407490.32017342606445404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1802330.23969286310586854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1233460.16403852730996246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1048830.13948448153852408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT951890.12659237734590514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCCCAGCACTTTGGGAGG929880.1236652552778265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA920630.12243509266402698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC816380.10857082753012433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT48036350.0131.660541
AGTGGTA48648500.0130.124575
GTGGTAT48735150.0129.920616
GGTATCA48772250.0129.871698
AGCAGTG48714750.0129.86892
GTATCAA48803700.0129.778649
TGGTATC48799950.0129.772167
CAGTGGT48956100.0129.358444
GCAGTGG49243350.0128.488863
CGCATAC59750.032.4906161
CATGGGG11722800.026.8139325-29
AGAGTAC48044300.026.46637715-19
CAACGCA48610200.026.4472210-14
CAGAGTA48123950.026.43501715-19
TACATGG47374000.026.35659820-24
GCAGAGT48205950.026.34751715-19
AGTACAT47903850.026.34741820-24
ATCAACG48705350.026.33438510-14
GTACATG47727750.026.31128320-24
ACATGGG47285900.026.30597120-24
Produced by FastQC (version 0.11.5)