FastQCFastQC Report
Tue 21 Mar 2023
SRR9602596_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602596_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111048366
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30752642.76930144113962No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1721390.1550126365659446No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1170480.1054027215492752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC5296450.0144.996031
ATAGCGA2914250.0144.356251
GTCGTAG2237300.0142.735521
GCGAGTA1802050.0141.831761
GATGTAC5169750.0141.307982
GCCACAT7401050.0141.166341
ATAGACA5478050.0140.273452
TAGCGAC3015950.0139.707532
GTGCGAA2338900.0139.365192
GTCTGTC2911600.0139.291281
AGAGATC3434700.0139.079882
TCGTAGA2311450.0137.920522
GGTGCGA2363250.0137.826111
AGATTCG921700.0136.312673
CCACATA7680500.0136.312352
CCGTCTA3560800.0136.259772
AGATGTA5356300.0136.2371
AGTTAGC1328950.0136.219972
AGCGACT1084100.0135.966353
TCACGCA2567900.0135.666292
Produced by FastQC (version 0.11.5)